FastQCFastQC Report
Sun 21 May 2023
EGAF00007832665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70934
Sequences flagged as poor quality0
Sequence length247-250
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA2683137.82530239377449No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2208731.137395325231903No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC52007.330758169566076No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA31284.4097329912312855No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC21623.0479036851157413No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG14922.1033636901908817No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC7241.0206670989934306No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT6100.8599543237375589No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC5040.7105196379733273No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG5030.7091098767868723No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC3740.5272506837341755No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC2940.41446978881777424No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC2170.3059181774607381No Hit
GGAAGTAAAAGTCGTAACAAGGTCAGTCGCACCGTAACTTCCGACAACCA2050.28900104322327796No Hit
GGAAGTAAAAGTCGTAACAAGGCAACGACGAGCACGATGCCGAGGATTTT1670.23543011813798742No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACTGAATCCGAAAAGAGCACA1630.22979107339216737No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG1560.21992274508698226No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT1350.19031776017142696No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC1330.18749823779851693No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1240.1748103871204218No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAGAACAGATAGGTGCGAATAATCAT1170.16494205881523671No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA1110.1564834916965066No Hit
GGAAGTAAAAGTCGTAACAAGGCGATGGGAAGTATGACGGAAGAGCTTAC1080.15225420813714158No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG880.12405898440804128No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA850.11982970084867624No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC810.11419065610285617No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC750.10573208898412609No Hit
GGAAGTAAAAGTCGTAACAAGGAGTACAGAGCGCTCGTGCAGTATCCCAA720.10150280542476103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)