FastQCFastQC Report
Sun 21 May 2023
EGAF00007832675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82929
Sequences flagged as poor quality0
Sequence length247-250
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA2835834.19551664677013No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC2177526.25740090921149No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC77449.338108502453906No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG41715.029603636845977No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG19502.3514090366458054No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC16431.9812128447225938No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG10071.2142917435396545No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA9161.1045593218295169No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC8711.05029603636846No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC8561.0322082745481074No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT7960.9598572272666981No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC7700.9285051067780872No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA6540.7886264153673624No Hit
GGAAGTAAAAGTCGTAACAAGGTACCCGAATGCCGGATTCCTCGCTAACA5130.6186014542560504No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC5100.6149839018919798No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT4360.5257509435782417No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA3760.45339989629683225No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA3150.3798429982273993No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC2780.3352265190705302No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC2670.3219621604022718No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT2600.3135212048861074No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC2350.28337493518552015No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC2100.2532286654849329No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC2010.24237600839272147No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1770.2134355894801577No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA1490.1796717674155No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT1450.17484836426340605No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA1390.1676132595352651No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC1250.15073134850293626No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGTGGCTGGAAC1200.1447020945628188No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG1140.13746698983467784No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC1040.12540848195444296No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGCTGGATC1020.12299678037839598No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1020.12299678037839598No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGAAACCTCTCCGGCA960.11576167565025505No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA870.10490901855804363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)