FastQCFastQC Report
Sun 21 May 2023
EGAF00007832691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88996
Sequences flagged as poor quality0
Sequence length247-250
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5136857.71944806508158No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1621218.216549058384647No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC19202.157400332599218No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGTGGCTGGAAC15031.6888399478628253No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG14521.6315340015281585No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC11431.2843273855004718No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC9381.0539799541552428No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC9051.016899635938694No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC7170.8056541867050204No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA6200.6966605240684974No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG5220.5865432154254123No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC4590.5157535170120006No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT3850.43260371252640567No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC3260.3663085981392422No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACTGGAAAGTGCGGCTGGATC2910.326980987909569No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC2700.30338442177176506No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT2630.2955188997258304No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG2590.291024315699582No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCTTAGGTGAACCTGCGGAAGGATC1990.22360555530585643No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCAGTAGGTGAACCTGCGGAAGGATC1610.18090700705649693No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC1430.16068137893837925No Hit
GGAAGTAAAAGTCGTAACAAGGCATGAGGTTGCAGGATATGGTGTTCATA1410.15843408692525507No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT1400.15731044091869295No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC1270.14270304283338578No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTATGAGTGCTGGAAGG1260.14157939682682366No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1250.1404557508202616No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1130.12697199874151646No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTACGGCA1120.12584835273495437No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1040.11685918468245765No Hit
GGAAGTAAAAGTCGTAACAAGGATGTGCATGTACCCATCGTAAAGCGAAG990.11124095464964717No Hit
GGAAGTAAAAGTCGTAACAAGGTACCCGAATGCCGGATTCCTCGCTAACA930.10449907861027463No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA910.10225178659715042No Hit
GGACGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC890.10000449458402626No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)