FastQCFastQC Report
Sun 21 May 2023
EGAF00007832712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81264
Sequences flagged as poor quality0
Sequence length235-238
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG4543155.90544398503643No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATTAAAATAA80199.867838157117543No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG53436.574867099822799No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT32193.9611636148848195No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT31803.913171884229179No Hit
GCTGCGTTCTTCATCGATGCCGCAATGGTAGGGCATTTAGGCTCAGCAGC28933.5600019688915143No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG10721.3191573144319748No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG9911.2194821815317975No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA6730.8281649931088797No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG6160.7580232329198662No Hit
CCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG3810.46884229178972237No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT3230.39746997440441034No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCATAGCCAAGTAGCAG3020.37162827328214215No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT2880.3544004725339634No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT2780.3420949005709785No Hit
GCTGCGTTCTTCATCGATGCCGGTCGCTTTGGAAACCGCCGTGCGGACAT2260.2781059263634574No Hit
GCTGCGTTCTTCATCGATGCGTGACTGTTGGGCCCCACTGCTGCGACTCT1700.20919472337074227No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG1610.19811970860405592No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA1470.18089190785587714No Hit
GCTGCGTTCTTCATCGATGCGTTGATATTCCACTTCGCAAATCAAAATAA1410.17350856467808623No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG1300.1599724355188029No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG1280.15751132112620594No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1160.14274463477062413No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG1110.13659184878913172No Hit
GCTGCGTTCTTCATCGACTCCTGACGCCAAGGCATCCACCGTATGCCTTT1070.13166962000393778No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT1070.13166962000393778No Hit
GCTGCGTTCTTCATCGATGCCAAGGAGCGGATTAAACCCTTCAGTAAGAC1030.12674739121874384No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG1000.12305571962984839No Hit
GCTGCGTTCTTCATCGATACCGGTCGCTTTGGAAACCGCCGTGCGGACAT940.1156723764520575No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG860.10582791888166963No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGATAGTCG820.1009056900964757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)