FastQCFastQC Report
Sun 21 May 2023
EGAF00007832737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115095
Sequences flagged as poor quality0
Sequence length247-250
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4532739.38224944610973No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1151510.004778661106043No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA63925.553673052695599No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG47544.13050089056866No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC44693.8828793605282597No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC36783.195621008731917No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC36503.1712932794647903No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTAGGATAAAGCA34412.989704157435162No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC25242.1929710239367477No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCTGAACTGACGTGGGAACAGGTCCG24022.0869716321299796No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC18261.586515487206221No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG18071.5700073852035275No Hit
GGAAGTAAAAGTCGTAACAAGGACAGTAAAGTCATCCCGACCCAACGCGA12461.0825839523871583No Hit
GGAAGTAAAAGTCGTAACAAGGTACGGACGCATCGATGAAGAACGCAGCC8610.74807767496416No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCAAGGTACG8320.722881098223207No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA7630.662930622529215No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT7010.6090620791520049No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC7010.6090620791520049No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA6710.5829966549372257No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG5630.489161127764021No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC5520.4796038055519354No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC5460.47439072070897953No Hit
GGAAGTAAAAGTCGTAACAAGGTCAAGCTCCTAAGGACAAAAAACAAGAT4570.3970632955384682No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGCCGCAATCG4130.35883400669012555No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA4100.35622746426864765No Hit
GGAAGTAAAAGTCGTAACAAGGTAAGCAAGTCAGGCAAAACCAAGTAACG4050.3518832268995178No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTATGAGTGCTGGAAGG3860.33537512489682436No Hit
GGAAGTAAAAGTCGTAACAAGGCACGTCGGCACACTGGAAGCCATAAGCT3250.2823754289934402No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT3010.2615230896216169No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC2740.23806420782831575No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG2420.2102610886658847No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGAAGGATC2100.18245796950345367No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC2060.17898257960814978No Hit
GGAAGTAAAAGTCGTAACAAGGATAATGCATTCAAGACATCCAGCCGATA2040.17724488466049784No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGATAATGAAGAAACAAAGGTTGGT1990.172900647291368No Hit
GGAAGTAAAAGTCGTAACAAGGGCTCTAATTAGACTCAGATGAAGAACGC1880.16334332507928231No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC1840.15986793518397846No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGTGGCATAGATGGTGATAACTTGTA1740.15117946044571876No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGACGTCGAGAAGGAAGCGGC1680.14596637560276293No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC1650.14335983318128503No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGAAGGAATTCAGAAGAGCCAGGGTG1610.13988444328598112No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA1500.1303271210738955No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC1380.11990095138798385No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA1360.11816325644033189No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC1340.11642556149267996No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCTGAACTGACGTGGGATCAGGTCCG1320.11468786654502802No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC1290.11208132412355012No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1260.1094747817020722No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1230.1068682392805943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)