FastQCFastQC Report
Sun 21 May 2023
EGAF00007832739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51281
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1035320.18876386965933No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC686513.387024434000896No Hit
GGAAGTAAAAGTCGTAACAAGGTCGAGCAGAACAAGCTCACCGTCACGGG32246.2869288820420826No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA28635.582964450771241No Hit
GGAAGTAAAAGTCGTAACAAGTCCTTGATCGGTGGCAGCGCCGCAATGGA23854.650845342329517No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG23434.568943663345099No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC16243.1668649207308746No Hit
GGAAGTAAAAGTCGTAACAAGAGATGGAGATTAGTCAATATTATCGAAAG15022.928960043680896No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGGTCAGCTAATCGACGCAATC14992.923109923753437No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC14802.8860591642128663No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG14632.852908484623935No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT9601.8720383767867241No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG9051.7647861781166514No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG5451.06277178682163No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA5251.0237709873052396No Hit
GGAAGTAAAAGTCGTAACAAGGTATGAGAAGAAGAGCCCAGGTGAGGCAA4370.8521674694331233No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG4270.8326670696749283No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA3550.692264191415924No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA3160.6162126323589634No Hit
GGAAGTAAAAGTCGTAACAAAGACAGAAATGAAGACAGTCAGAAATGAAG2900.5655115929876563No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT2200.4290087946802909No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG2110.4114584348979154No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGGCATGAAACTCACTGAAAGCTAC1950.3802577952848033No Hit
GGAAGTAAAAGTCGTAACAAGGGATGGAGATTAGTCAATATTATCGAAAG1860.3627074355024278No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC1800.3510071956475108No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1780.34710711569587177No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1510.2944560363487452No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1460.2847058364696476No Hit
GGAAGTAAAAGTCGTAACAAAGCAAAAGACGGCACGACGGATGCGATGAT1450.28275579649382815No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC1410.2749556365905501No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1380.2691055166630916No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGGAGAGAAACGATGAGCCTGGAT1310.2554552368323551No Hit
GGAAGTAAAAGTCGTAACAAGGCTGCGGACAGGGAGACAGAGAGTAAGAG1280.24960511690489653No Hit
GGAAGTAAAAGTCGTAACAAGGTGCCCAAATGAAGCACGCCGGGGCGGAC1280.24960511690489653No Hit
GGAAGTAAAAGTCGTAACAAGGCCAAGAGGAAAACAACGAGAGGGCAAGA1180.23010471714670153No Hit
GGAAGTAAAAGTCGTAACAAGGGAACCGCTTTTACGCAGGAGGAGCGCCA1150.224254597219243No Hit
GGAAGTAAAAGTCGTAACAAGGTGGGTAAGGAGCTCCTGGACGCGCTCCC1130.220354517267604No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGATGTGACCGACAAGTCCAAGAAG1130.220354517267604No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA1110.21645443731596498No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1080.21060431738850643No Hit
GGAAGTAAAAGTCGTAACAAGGGGATAAGTATCGGCTACCTCCCGCAGGT1070.20865427741268697No Hit
GGAAGTAAAAGTCGTAACAAGGACAGAAATGAAGACAGTCAGAAATGAAG1060.20670423743686744No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGCAGTTGTTTGGATACGCCGCGCGT1060.20670423743686744No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGCTGGATC1000.19500399758195044No Hit
GGAAGTAAAAGTCGTAACAAGACGAAGAAGTACCATGCTCACGACGAGAA960.1872038376786724No Hit
GGAAGTAAAAGTCGTAACAAGGCGCGATTTTGCGCGAAGGCATGAACATC920.1794036777753944No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA890.17355355784793589No Hit
GGAAGTAAAAGTCGTAACAAGGAATCATCATGCAGTGAGGAAGGATCATG870.16965347789629687No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA860.16770343792047737No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGCATCAGGAGACCAATAGGGTCGAT840.16380335796883835No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG820.15990327801719934No Hit
GGAAGTAAAAGTCGTAACAAGGATGAAAACTCCAGCTTCAACGTCACGCT820.15990327801719934No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA770.15015307813810183No Hit
GGAAGTAAAAGTCGTAACAAGGGATAAAAGTTGTAGGAAGAGCAAGCAGG750.1462529981864628No Hit
GGAAGTAAAAGTCGTAACAAGGAGACCGAGGAGAAGAAATCCGATAAGAC740.14430295821064332No Hit
GGAAGTAAAAGTCGTAACAAGGCACGGCGCAAGAGCGGAGAAATACGGCC720.1404028782590043No Hit
GGAAGTAAAAGTCGTAACAAGAAGAGCATCAGGAGACCAATAGGGTCGAT690.1345527583315458No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGTGGACATCCGCGTGGCGATCAG690.1345527583315458No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC680.1326027183557263No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC670.1306526783799068No Hit
GGAAGTAAAAGTCGTAACAAGGCACTGGAAGGTTCAGGCTGCCCACAATC650.12675259842826778No Hit
GGAAGTAAAAGTCGTAACAAGTGCGAGCGAATCGGCAAGTACGTCAGCGT640.12480255845244827No Hit
GGAAGTAAAAGTCGTAACAAGGGAATAAGTATCGGTTACCTGCCGCAGGT630.12285251847662876No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC630.12285251847662876No Hit
GGAAGTAAAAGTCGTAACAAGGCTGGTGGTGAGCAGCATGGCACATAAAA620.12090247850080928No Hit
GGAAGTAAAAGTCGTAACAAGGGTACGGGATGGGCTTACCCGCTCAGGCT590.11505235857335076No Hit
GGAAGTAAAAGTCGTAACAAGCGGTAAGAAGAATACGATTAGCACTTGAC590.11505235857335076No Hit
GGAAGTAAAAGTCGTAACAAGGAGGCATTAATTATGAAAAAAACAGGCGA550.10725219867007273No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGGCGGGTTGCCGGACTCTATCCGGG540.10530215869425322No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC530.10335211871843372No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG520.10140207874261423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)