FastQCFastQC Report
Sun 21 May 2023
EGAF00007832785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67475
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2356134.918117821415336No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1912028.33642089662838No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC816612.102260096331975No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG30754.557243423490181No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC15012.224527602815858No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG11761.74286772878844No Hit
GGAAGTAAAAGTCGTAACAAGGTTCCAGACCGAAGAGATAGAGCTGCGAT7501.1115227862171175No Hit
GGAAGTAAAAGTCGTAACAAGGCCGGAGATAGCGTAAGCGCGTTGACAGC6510.964801778436458No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC4450.6595035198221564No Hit
GGAAGTAAAAGTCGTAACATGAGTGCAGGCTTCCAGGAACTGGCAACAGG4250.6298629121896998No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT3740.5542793627269359No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC3710.5498332715820674No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCGTCGTTACC3140.46535753982956646No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGAATACGACGAGAGGCGACATCCT2550.37791774731381994No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC2190.32456465357539827No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC2120.3141904409040385No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC1870.27713968136346795No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1860.2756576509818451No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1850.2741756206002223No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT1820.26972952945535383No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1700.25194516487588No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGATAATGAAGAAACAAAGGTTGGT1590.23564283067802888No Hit
GGAAGTAAAAGTCGTAACAAGGTCATCCACCTTTATCCCGACCGTGCCGG1560.23119673953316042No Hit
GGAAGTAAAAGTCGTAACAAGGCACGTCAGCACACTGGAAGCCATAAGCT1320.19562801037421268No Hit
GGAAGTAAAAGTCGTAACAAGGCGAGGACAAGCGGTGAGATAACCGATGC1260.18673582808447572No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1140.16895146350500184No Hit
GGAAGTAAAAGTCGTAACAAGAGTGCAGGCTTCCAGGAACTGGCAACAGG1050.15561319007039645No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1040.1541311596887736No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC910.13486476472767692No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC870.12893664320118564No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG780.11559836976658021No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGCCGCAATCG680.10077806595035198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)