FastQCFastQC Report
Sun 21 May 2023
EGAF00007832807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65459
Sequences flagged as poor quality0
Sequence length247-250
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2178133.274263279304606No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1182618.06627048992499No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA53078.107364915443254No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC50837.7651659817595755No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC45486.947860492827572No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG34605.285751386363984No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC29254.46844589743198No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA17622.6917612551368033No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC9541.457400815777815No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG8101.2374157869811637No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT2760.42163797186024843No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT2270.34678195511694343No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT2010.3070624360286591No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1870.28567500267342916No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1860.2841473288623413No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCTTAGGTGAACCTGCGGAAGGATC1720.2627598955071113No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT1700.25970454788493563No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC1470.2245680502299149No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC1400.2138743335522999No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT1280.19554224781924562No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCGTAACCGAA1270.19401457400815778No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1250.19095922638598206No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT1170.1787378358972792No Hit
GGAAGTAAAAGTCGTAACAAGGAGAAATGATGTCGATCAAGAAGATTGCG1110.16957179303075207No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC990.15123970729769778No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC980.14971203348660994No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC950.14512901205334636No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA880.13443529537573137No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC840.12832460013137995No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG770.11763088345376495No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGATAATGAAGAAACAAAGGTTGGT770.11763088345376495No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC740.11304786202050138No Hit
GGAAGTAAAAGTCGTAACAAGGGTGTCTTAGACGCACACGACAGGGTCTT700.10693716677614995No Hit
GGAAGTAAAAGTCGTAACAAGGTGCCCAAATGAAGCACGCCGGGGCGGAC690.10540949296506211No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA680.10388181915397424No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA660.10082647153179852No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)