FastQCFastQC Report
Sun 21 May 2023
EGAF00007832845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63834
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1720026.9448883040386No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1584424.820628505185326No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG1053216.49904439640317No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGGTGTGGTGTTCCAAGCAAGGCAGG48587.610364382617414No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA22703.5560986308236986No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG21743.4057085565686No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC21603.3837766707397314No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC3150.49346743114954417No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA2910.45586991258576937No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC2760.43237146348341016No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT2740.4292383369364289No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC2290.35874298962935114No Hit
GGAAGTAAAAGTCGTAACAAGGGTAGGTAGAGATGAAGAACGCAGCCAGC2120.33211141398001065No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC2080.3258451608860482No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1970.30861296487765144No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1800.28198138922831095No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC1790.2804148259548203No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC1620.2537832503054798No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC1540.24125074411755493No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGATAATGAAGAAACAAAGGTTGGT1540.24125074411755493No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGGTCAGCTAATCGACGCAATC1410.2208854215621769No Hit
GGAAGTAAAAGTCGTAACAAGGATGTGCATGTACCCATCGTAAAGCGAAG1340.20991947864774257No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT1240.19425384591283643No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1170.1832879029984021No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC1160.1817213397249115No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1140.17858821317793028No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT1080.16918883353698658No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAACCAACATGACTGATGAAGAAAAA1020.15978945389604288No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA1010.15822289062255226No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGTGGCATAGATGGTGATAACTTGTA990.155089764075571No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCAGCCTATC930.1456903844346273No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG870.1362910047936836No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT870.1362910047936836No Hit
GGAAGTAAAAGTCGTAACAAGGCGTTAATCGTACATAAATACGGCGGCAC720.11279255569132438No Hit
GGAAGTAAAAGTCGTAACAAGGATAAGAATGAGAGATGAAATATTCCACT640.10026004950339945No Hit
GGAAGTAAAAGTCGTAACAAGGTCAAGCTCCTAAGGACAAAAAACAAGAT640.10026004950339945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)