FastQCFastQC Report
Sun 21 May 2023
EGAF00007832854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79695
Sequences flagged as poor quality0
Sequence length235-238
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT3644945.735617039964865No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT2167527.197440240918503No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG38964.888637932116193No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG27693.474496517974779No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT26993.3866616475312123No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG7450.9348139782922392No Hit
GCTGCGTTCTTCATCGATGCGTGACTGTTGGGCCCCACTGCTGCGACTCT7230.9072087332956897No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA6080.7629085889955456No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG5010.6286467156032374No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG4860.6098249576510446No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTCGCAAATCA4000.5019135453918063No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT3290.4128238910847607No Hit
GCTGCGTTCTTCATCGATGCCTCTGCTATCCTGGCTGCGTTCTTCATCGA3250.4078047556308426No Hit
GCTGCGTTCTTCATCGATGCCTCTGCTATCCTCGCTGCGTTCTTCATCGA2260.2835811531463705No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATTAAAATAA2200.27605244996549344No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC2170.2722880983750549No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2130.26726896292113683No Hit
GCTGCGTTCTTCATCGATGCACGATCATTCCTGGTTGCACGATCCTTCCT2100.2635046113306983No Hit
GCTGCGTTCTTCATCGATGCCTCCCCAGTATCTCCTGCTCTCTTTTGCAT2070.2597402597402597No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT2020.25346634042286215No Hit
GCTGCGTTCTTCATCGATGCACGATCCTTCCTCGCTGCAGGATCCTTCCT1920.24091850178806698No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG1860.23338979860718992No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTAAAAGTTG1830.22962544701675136No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1570.19700106656628394No Hit
GCTGCGTTCTTCATCGATGCCAAGGAGCGGATTAAACCCTTCAGTAAGAC1550.19449149883932493No Hit
GCTGCGTTCTTCATCGATGCCTCTGCTATCCTTGCTGCGTTCTTCATCGA1510.18947236338540688No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT1410.17692452475061168No Hit
GCTGCGTTCTTCATCGATGCAGGATCCTTCCTCGCTGCACGATCATTCCT1310.16437668611581654No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC1260.15810276679841898No Hit
TCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT1140.14304536043666477No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTTGCAAATTA900.1129305477131564No Hit
GCTGCGTTCTTCATCGATGCATTCAATATTGCTATTTACTTTGCAAATTA890.11167576384967691No Hit
GCTGCGTTCTTCATCGATGCCTCTGCTATCCTAGCTGCGTTCTTCATCGA840.10540184453227931No Hit
TCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT810.10163749294184077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)