FastQCFastQC Report
Sun 21 May 2023
EGAF00007832859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124999
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5849246.793974351794816No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG2196217.56974055792446No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT59304.744037952303618No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA48713.896831174649397No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC48503.880031040248322No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG45313.624828998631989No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC16901.3520108160865287No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGCAGTCGGGGTACCAGTGCCCAGA13751.1000088000704007No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC13741.0992087936703494No Hit
GGAAGTAAAAGTCGTAACAAGGAATCATCATGCAGTGAGGAAGGATCATG12811.0248081984655877No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG12551.0040080320642564No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAGCTGGCATCTCAACAATGACGG11400.9120072960583685No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG8830.7064056512452099No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG8630.6904055232441859No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA8440.6752054016432131No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA7650.6120048960391683No Hit
GGAAGTAAAAGTCGTAACAAGGTAAGAAAAATAAATAAAATTTAAAAACC7580.6064048512388098No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC4950.39600316802534424No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCAGGGCTTCGACCTTCATGCCGCGT4660.3728029824238594No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC4660.3728029824238594No Hit
GGAAGTAAAAGTCGTAACAAGGACCCTCCTTCCCCTGAGTCCTGCAGTTT3750.30000240001920014No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGAGCTGGCTCCCCTGCACACAATCA3740.29920239361914897No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT3670.29360234881879055No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGGCAAGAAGCTCCACCTCACCCCAG3160.25280202241617933No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA2680.2144017152137217No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGAAGGAATTCAGAAGAGCCAGGGTG2630.2104016832134657No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGGTGGCGTCTCTGAGGGCAAAGCT2300.18400147201177608No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGCTACCAAAGCTCCTCCCTCTTCA2220.1776014208113665No Hit
GGAAGTAAAAGTCGTAACAAAGTTTTGGTAGGTGAACCTGTGGAGGGATC2160.1728013824110593No Hit
GGAAGTAAAAGTCGTAACAAGGAAATGCGGATCCTCATGTTGGGCCTGGA2090.16720133761070088No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1980.15840126721013767No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1980.15840126721013767No Hit
GGAAGTAAAAGTCGTAACAAGGCGGAGTGGGAGGCCTACAGCGCCGGGAT1950.15600124800998408No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1900.15200121600972807No Hit
GGAAGTAAAAGTCGTAACAAGGTGGGTAAGGAGCTCCTGGACGCGCTCCC1740.13920111360890886No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGTAAGGATC1620.1296010368082945No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAATAAGGACAGATCCATTAGGAGGC1380.11040088320706566No Hit
GGAAGTAAAAGTCGTAACAAGGACCTTCAAAAGGGGAGCGCGCGGAAGTG1320.10560084480675845No Hit
GGAAGTAAAAGTCGTAACTAAGAATGGGTTGCCTGCCAGCCCTGGCAGGT1250.10000080000640006No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)