FastQCFastQC Report
Sun 21 May 2023
EGAF00007832861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66124
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2844143.01161454237493No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1125617.022563668259632No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG777011.75065029338818No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG769911.643276268828263No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC10521.5909503357328654No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT7081.0707156251890388No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC5720.8650414372996188No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC5020.7591797229447704No Hit
GGAAGTAAAAGTCGTAACAAGGCGGAGTTGCACAAACTCTTTTTTAACTT4450.6729780412558224No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC4170.630633355513883No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC3070.464279232956264No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG2940.44461920029036356No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA2910.4400822696751558No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT2650.4007622043433549No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGTCAGCCAATCGCCGCAATCG2000.30246204101385277No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC1950.29490048998850643No Hit
GGAAGTAAAAGTCGTAACAAGGCGTGGGGTACGTTCGGTGCTGGTCTGCT1840.2782650777327445No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG1830.27675276752767525No Hit
GGAAGTAAAAGTCGTAACAAGGTACCCGAATGCCGGATTCCTCGCTAACA1480.22382191035025104No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTCAC1290.19508801645393503No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1220.18450184501845018No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC1080.1633295021474805No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1030.15576795112213418No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGCAGTCGGGGTACCAGTGCCCAGA960.14518177968664933No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG960.14518177968664933No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTATCCGGCA950.14366946948158008No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC950.14366946948158008No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGAGGAAGTG900.13610791845623374No Hit
GGAAGTAAAAGTCGTAACAAGGAGATCCGGAACAAGGAGATCGAGAACAA880.1330832980460952No Hit
GGAAGTAAAAGTCGTAACAAGGAGATCGAGAACAAGGAGGTTTTGCCATG870.13157098784102594No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT780.11796019599540257No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC770.1164478857903333No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCAGTAGGTGAACCTGCGGAAGGATC740.11191095517512552No Hit
GGAAGTAAAAGTCGTAACAAGGCAATGAAACAAGCATACAACAAAAAATG720.10888633476498699No Hit
GGAAGTAAAAGTCGTAACAAGGAGAAATGATGTCGATCAAGAAGATTGCG700.10586171435484847No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)