FastQCFastQC Report
Sun 21 May 2023
EGAF00007832862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66124
Sequences flagged as poor quality0
Sequence length235-238
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT1094116.546185953662814No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1072316.216502328957716No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG767411.605468513701531No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT749411.333252676789062No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT670610.141552235194483No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG51197.741515939749561No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG35275.333918093279293No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT9471.4321577642005927No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC6811.0298832496521686No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA5150.7788397556106709No Hit
GCTGCGTTCTTCATCGATGCGTACATAAACTATGTAGGAACGCCCGCGCT4720.7138104167926925No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT4510.682051902486238No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA3760.5686286371060432No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT3250.4915008166475107No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT3000.45369306152077915No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG2960.44764382070050207No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2930.4431068900852943No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT2760.4173976165991168No Hit
GCTGCGTTCTTCATCGATGCGTGACTGTTGGGCCCCACTGCTGCGACTCT2630.39773758393321634No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG2290.3463190369608614No Hit
GCTGCGTTCTTCATCGATGCTGTGGTGGTGACGGTGGCTGCTGCGCTGGG2070.3130482124493376No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCATTGTTAAAAGTTG1920.29036355937329866No Hit
GCTGCGTTCTTCATCGATGCGTCGGACAGTCACAATCAACTCACATGCGG1880.2843143185530216No Hit
GCTGCGTTCTTCATCGATGCCGGTCGCTTTGGAAACCGCCGTGCGGACAT1870.2828020083479523No Hit
GCTGCGTTCTTCATCGATGCCTGGGTTCCCGTCAACACCAGGAACATCGC1750.26465428588712114No Hit
GCTGCGTTCTTCATCGACTCCTGACGCCAAGGCATCCACCGTATGCCTTT1730.26162966547698263No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA1630.24650656342629No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT1590.24045732260601296No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG1540.2328957715806666No Hit
GCTGCGTTCTTCATCGATGCCAAGGAGCGGATTAAACCCTTCAGTAAGAC1500.22684653076038958No Hit
GCTGCGTTCTTCATCGACTCGAAACGCCTAGGCATCCACCGTATGCCCTT1470.22230960014518178No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC1360.20567418788941988No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT1250.18903877563365798No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT1220.18450184501845018No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTCGCAAATCA1160.1754279837880346No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG1110.16786643276268828No Hit
GCTGCGTTCTTCATCGATACCGGTCGCTTTGGAAACCGCCGTGCGGACAT1030.15576795112213418No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG1020.1542556409170649No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGT1000.15123102050692638No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG990.14971871030185713No Hit
GCTGCGTTCTTCATCGACCCGAAACGCCTAGGCATCCACCGTATGCCCTT940.1421571592765108No Hit
GCTGCGTTCTTCATCGACACGAGAGCCAAGATATCCATTGCCAGGAGTCA910.13762022866130302No Hit
GCTGCGTTCTTCATCGATGCGCACACTACTCCCACCGCCCCCGAGTGCCT900.13610791845623374No Hit
GCTGCGTTCTTCATCGATGCCTTTTCCGAGGCCGGACGATCGGGCAAACT850.12854636743088743No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCTAGGCCGGACGATCGGGCAAACT770.1164478857903333No Hit
GCTGCGTTCTTCATCGATGCAGAGCATACCGTTGTCACCGTTCCTCCGGC760.11493557558526406No Hit
GCTGCGTTCTTCATCGCTGTCAGATGCCTTGGCATCCTTCGTATGCCCTT760.11493557558526406No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCTGGGAAAGTCA740.11191095517512552No Hit
GCTGCGTTCTTCATCGATGCGGTACCGACTTCGAAGACTTGCAGGATGGC690.1043494041497792No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAATCGTACTCCACAACAGG690.1043494041497792No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAATTTT670.10132478373964067No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)