FastQCFastQC Report
Sun 21 May 2023
EGAF00007832890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149303
Sequences flagged as poor quality0
Sequence length237-238
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG11368076.14046603216278No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT39652.65567336222313No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG34512.3114070045477986No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT31142.0856915132314824No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT30122.0173740648212024No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA29691.988573571863928No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT25661.7186526727527245No Hit
GCTGCGTTCTTCATCGATGCGTTACGTATTCGGCTGCGTTCTTCATCGAT17441.1680944120345875No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC5830.3904811021881677No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT5770.3864624287522689No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG4290.2873351506667649No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT4030.26992089911120337No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG3760.25183686864965876No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT3710.24848797411974308No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT3080.2062919030428056No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG2960.19825455617100796No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCTGAGAGTCG2440.16342605305988495No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG2180.14601180150432344No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT2060.1379744546325258No Hit
GCTGCGTTCTTCATCGATGCCGGTGAATTATGCGATGGTGGTGGCGGCGG2040.13663489682055954No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT1960.13127666557269443No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1950.1306068866667113No Hit
GCTGCGTTCTTCATCGATGCGTTGATATTCCACTTCGCAAATCAAAATAA1890.1265882132308125No Hit
GCTGCGTTCTTCATCGATGCGAGAGCTGAGATATCCGTTGCCGAGAGTCG1890.1265882132308125No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGCCGAGAGTCG1850.12390909760687997No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG1780.11922064526499804No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG1710.11453219292311607No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC1690.11319263511114981No Hit
GCTGCGTTCTTCATCGATGCGTTACGTATTCGGCTGCGTTCTTCATCGGC1630.109173961675251No Hit
GCTGCGTTCTTCATCGATGCGAGAGCAGAGATATCCGTTGCCGAGAGTCG1580.10582506714533532No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG1580.10582506714533532No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCTGTTGCCGAGAGTCG1560.10448550933336906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)