FastQCFastQC Report
Tue 7 May 2024
EGAF00007832907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74961
Total Bases18.6 Mbp
Sequences flagged as poor quality0
Sequence length247-250
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC3624348.34914155360788No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA59717.965475380531209No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA49206.563412974746869No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC21912.9228532170061765No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC21062.8094609196782327No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG17972.397246568215472No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAAAGGACCGGAATCTGCTTCATACG13851.847627432931791No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC10921.4567575139072317No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT10891.4527554328250691No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT9331.2446472165526075No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAGTGGAACTTTGCAGGGGATGAAAA8581.1445951894985393No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC8551.1405931084163765No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA7811.041875108389696No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC7340.97917583810248No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAGAAGGACTACAGAAAGAAACAGGG4790.6389989461186484No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC4620.6163204866530596No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA3290.4388948920105122No Hit
GGAAGTAAAAGTCGTAACAAGGAGTTTAGGTCGGCACTAACTGCACTTGG2810.3748615946959085No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCGATGGTAGTTG2730.36418937847680793No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC2400.320166486573018No Hit
GGAAGTAAAAGTCGTAACAAGGATTTGCGCAGGTTGAAGGTGGTGTGCGC2370.3161644054908552No Hit
GGAAGTAAAAGTCGTAACAAGCCCACAGGATTTAGTATCGATGGTAGTTG2340.3121623244086925No Hit
GGAAGTAAAAGTCGTAACAAGGAACTGGCGACGTGCTTCAGGACTGCCTA2160.28814983791571613No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA2100.28014567575139077No Hit
GGAAGTAAAAGTCGTAACAAGGGAGAAAACGGTGAATGTGAGCGAAGAAG1890.2521311081762517No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACTGGAAAGTGCGGCTGGATC1680.22411654060111255No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT1640.2187804324915623No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1620.21611237843678716No Hit
GGAAGTAAAAGTCGTAACAAGGCAACGACGAGCACGATGCCGAGGATTTT1540.20544016221768652No Hit
GGAAGTAAAAGTCGTAACAAGGTCGTCTAAATCGATGGTGCGGCTGATCT1540.20544016221768652No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1430.19076586491642322No Hit
GGAAGTAAAAGTCGTAACAAGAGGCTGAGTACACACACTACGAAACTCAA1290.17208948653299716No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGAGACGATGAGCGTTGATGCAGCGT1290.17208948653299716No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA1230.1640853243686717No Hit
GGAAGTAAAAGTCGTAACAAGGTGTTGCCGTCTTTGTCGGTAACATCTTT1160.15474713517695868No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC1160.15474713517695868No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTTCCAAAAGGGGTTCTCAGAGCTTC1140.15207908112218352No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC1130.15074505409479597No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC1120.1494110270674084No Hit
GGAAGTAAAAGTCGTAACAAGGGAGATGACTGCGGCACCGGCGTTGCGTT1090.14540894598524567No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1060.14140686490308293No Hit
GGAAGTAAAAGTCGTAACAAGGACAGAGCAACGTATCAGGCGGCGGTGGC1030.13740478382092022No Hit
GGAAGTAAAAGTCGTAACAAGGGCATTAAGGGTCGGCAGCGCCGCTTCTG950.1267325676018196No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA920.12273048651965689No Hit
GGAAGTAAAAGTCGTAACAAGGGCGATGTGAAGACCAGATGCAATGCCAG910.1213964594922693No Hit
GGAAGTAAAAGTCGTAACAAGGAGAAATGATGTCGATCAAGAAGATTGCG910.1213964594922693No Hit
GGAAGTAAAAGTCGTAACAAGAAATGATAATTTAAACAAAAATACAGCTA900.12006243246488174No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC870.116060351382719No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG860.11472632435533144No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA830.11072424327316871No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC820.10939021624578114No Hit
GGAAGTAAAAGTCGTAACAAGGGCGTGGGGCAGAAGGTGGAAAATGCGCG810.10805618921839358No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC770.10272008110884326No Hit
GGAAGTAAAAGTCGTAACAAGGGCGACGATAGAAGAGACCAGGAAGGCAG760.10138605408145569No Hit
GGAAGTAAAAGTCGTAACAAGGACGGCATCATTGGTTACGTTCTTGCAAT750.10005202705406811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)