FastQCFastQC Report
Sun 21 May 2023
EGAF00007832914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81010
Sequences flagged as poor quality0
Sequence length235-238
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG3950948.7705221577583No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1033712.760153067522529No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT50866.278237254659919No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT28853.5612887297864457No Hit
GCTGCGTTCTTCATCGATGCTACCCTTATCTGCAGCAGCTGGAATCTCTG16652.0553018145907913No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT5650.6974447599061845No Hit
GCTGCGTTCTTCATCGATGCCCGCATGCCTGTTCGTCGCTACGAGGTGCT5470.6752252808295273No Hit
GCTGCGTTCTTCATCGATGCACCTTTCAATCTTAAATATTACAAAAAGGG5350.6604122947784224No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT5180.639427231206024No Hit
GCTGCGTTCTTCATCGATGCTGCCAAAAACGTCTTACAGAACTGCTAACT4590.5665967164547586No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT4520.5579558079249475No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTTGTCATTTACTACAAACAAATTTC3950.4875941241821997No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG3880.4789532156523886No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG3530.435748673003333No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA3280.4048882853968646No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT2990.3690902357733613No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC2950.3641525737563264No Hit
GCTGCGTTCTTCATCGATGCCAGCGCTGCGGGGTCGGGGATATCGGCGAG2880.3555116652265153No Hit
GCTGCGTTCTTCATCGATGCCTCGTCGTAATCTCCATAGCCGTGCAAACT2670.32958893963708186No Hit
GCTGCGTTCTTCATCGATGCCCTCTATCAGACGAATGGTAAAGGGGATAT2590.319713615603012No Hit
GCTGCGTTCTTCATCGATGCGGTATAAAACCTGATCCTTGGTGACATGGT2540.3135415380817183No Hit
GCTGCGTTCTTCATCGATGAAGTCGAGAGAAATTTGTTCTTGTCCGAGGT2510.3098382915689421No Hit
GCTGCGTTCTTCATCGATGCGGAGCTTGACGTCGCGTGGGCAGATCATCC2440.30119738303913096No Hit
GCTGCGTTCTTCATCGATGCCACGAAGGTCGAGAATCTGGAGCTGATCGG1980.24441426984322923No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGATATCCGTTGTTAAGAGTCG1920.23700777681767682No Hit
GCTGCGTTCTTCATCGATGCCCACCAAGCGCCCCCGCGTGGAAAGGCGAT1860.2296012837921244No Hit
GCTGCGTTCTTCATCGATGCAGTGCTTGTCATCTACTACAAACAAATTTC1820.22466362177508947No Hit
GCTGCGTTCTTCATCGATGCTGCCCACGGTTTGGGCATACGCGTACTGCT1730.21355388223676092No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1730.21355388223676092No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG1570.19380323416862116No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC1540.19009998765584496No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA1420.17528700160474017No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG1360.16788050857918776No Hit
GCTGCGTTCTTCATCGATGCCGACGAATATGCGCAAAACCCCGGCCTGCA1350.16664609307492903No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT1300.16047401555363536No Hit
GCTGCGTTCTTCATCGATGCGGATTATCGGGGAGAAATCGGCGTAATCTT1270.15677076904085915No Hit
GCTGCGTTCTTCATCGATGCGAGAGCAGAGATATCCGTTGCCGAGAGTCG1250.15430193803234168No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCTTTGCCGAGAGTCG1220.15059869151956548No Hit
GCTGCGTTCTTCATCGATGCGAGAGACGAGATATCCGTTGCCGAGAGTCG1210.14936427601530675No Hit
GCTGCGTTCTTCATCGATGCCGAGCATGCTTTTGACCCGATTTACGCCCG1200.148129860511048No Hit
GCTGCGTTCTTCATCGATGCGATAGCCGAGATATCCGTTGCCGAGAGTCG1190.14689544500678928No Hit
GCTGCGTTCTTCATCGATGCGACCGAACTCGATCTCTTCCAGCTCTTCCT1100.13578570546846067No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCAGAGAGTCG1100.13578570546846067No Hit
GCTGCGTTCTTCATCGATGCAACACCGAAGACTATCCGCAGCTCGCACGT1090.13455128996420193No Hit
GCTGCGTTCTTCATCGATGCGAGATCCGAGATATCCGTTGCCGAGAGTCG1090.13455128996420193No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1090.13455128996420193No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATACGTTGCCGAGAGTCG1080.13331687445994322No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGACGAGAGTCG1070.1320824589556845No Hit
GCTGCGTTCTTCATCGATGCGGATACGCTGAGCACCGTGCCGCCGGACAA1060.13084804345142576No Hit
GCTGCGTTCTTCATCGATGCAATAAGATGTCCACCGTGGTCGGTTGATAG1060.13084804345142576No Hit
GCTGCGTTCTTCATCGATGCGTTGTCGTTTTGTTTTCAAGAAACTGGACA1040.12837921244290829No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT1030.12714479693864955No Hit
GCTGCGTTCTTCATCGATGCTAGAGCCGAGATATCCGTTGCCGAGAGTCG1020.12591038143439082No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCTAGGCCGGACGATCGGGCAAACT980.12097271941735588No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCTGACGATCGGGCAAACT940.11603505740032094No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCTAGAGTCG940.11603505740032094No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGATTCG920.11356622639180347No Hit
GCTGCGTTCTTCATCGCTGTCAGATGCCTTGGCATCCTTCGTATGCCCTT870.10739414887050983No Hit
GCTGCGTTCTTCATCGATGCACTCAACAGTTGGCAACTTGTCAAGGAACT870.10739414887050983No Hit
GCTGCGTTCTTCATCGATGCCGCCAAAATCGGCAGGGAACTGGGCTGGAA860.10615973336625109No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)