FastQCFastQC Report
Sun 21 May 2023
EGAF00007832924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82685
Sequences flagged as poor quality0
Sequence length235-238
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG3089337.36227852693959No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT1996224.142226522343833No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT1274515.413920299933482No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT27543.3307129467255248No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG18462.2325693898530568No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG15391.8612807643466167No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG8751.0582330531535344No Hit
GCTGCGTTCTTCATCGATGCGATAAAGATTGGCATCATGTTTAATGGGTG5670.6857350184434904No Hit
GCTGCGTTCTTCATCGATGCCATGAACGAGATTTCGGGAGCAATTATCTC5210.6301021950777045No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT4320.5224647759569451No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT4280.5176271391425289No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA4080.4934389550704481No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT3930.4752978170163875No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT2630.31807462054786234No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2610.3156558021406543No Hit
GCTGCGTTCTTCATCGATGCCTCTGCTATCCTGGCTGCGTTCTTCATCGA2130.2576041603676604No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2040.24671947753522405No Hit
TCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1600.1935054725766463No Hit
GCTGCGTTCTTCATCGATGCCTCTGCTATCCTCGCTGCGTTCTTCATCGA1490.1802019713370019No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA1440.1741549253189817No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1230.14875733204329683No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1210.14633851363608877No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1200.14512910443248472No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTAAAAGTTG1160.14029146761806857No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG1080.13061619398923627No Hit
GCTGCGTTCTTCATCGATGCCTCTGCTATCCTTGCTGCGTTCTTCATCGA1040.12577855717482012No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTCGAAAGTTG1040.12577855717482012No Hit
GCTGCGTTCTTCATCGATCGTCTTCCTTATTCTCAGACAAAATAAACTTC1030.12456914797121606No Hit
GCTGCGTTCTTCATCGATGCGATAGCCGAGATATCCGTTGCCGAGAGTCG950.11489387434238374No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCATTGCTGAAAGTTG930.11247505593517566No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG930.11247505593517566No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGTCCGGACGATCGGGCAAACT920.11126564673157163No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT920.11126564673157163No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCTTTGCCGAGAGTCG920.11126564673157163No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTA910.11005623752796759No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGTCGAGAGTCG850.10279978230634335No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCGAGATATCCGTTGCCGAGAGTCG830.10038096389913527No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACTATCGGGCAAACT830.10038096389913527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)