FastQCFastQC Report
Sun 21 May 2023
EGAF00007832925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80452
Sequences flagged as poor quality0
Sequence length247-250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4559456.67230149654452No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1482818.43086560930741No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA42465.277681101774971No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG22182.75692338288669No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT21562.6798587977924724No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG19052.367871525878785No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGTGGCTGGAAC11041.37224680554865No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC5160.6413762243325213No Hit
GGAAGTAAAAGTCGTAACAAGGGGTATTAAAGCCGACTGCAACCACCTTA5000.6214885894694974No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC3190.3965097200815393No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA2940.36543529060806446No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC2460.30577238601899265No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC1990.24735245860885996No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC1650.2050912345249341No Hit
GGAAGTAAAAGTCGTAACAAGGACGAGGTGGACATCCGCGTGGCGATCAG1650.2050912345249341No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1510.1876895540197882No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1460.18147466812509322No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA1020.12678367225177747No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT950.11808283199920448No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC890.11062496892557054No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC880.10938199174663153No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC870.10813901456769254No Hit
GGAAGTAAAAGTCGTAACAAGGAACTGTTGAAAAAGCTGGTAATTGTCAT820.10192412867299756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)