FastQCFastQC Report
Sun 21 May 2023
EGAF00007832929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79462
Sequences flagged as poor quality0
Sequence length247-250
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4329054.47887040346329No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTCAC57317.212252397372329No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG57287.208477007877979No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG44255.568699504165513No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT43825.514585588079838No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG15701.975787168709572No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC15041.892728599833883No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC14471.8209961994412425No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC13181.658654451184214No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC6750.8494626362286376No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC6270.7890564043190456No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC5760.7248747829151041No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT3760.47318214995847074No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC3580.4505298129923737No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT2870.3611789282927689No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG2350.2957388437240442No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC2310.29070499106491154No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGAGAAACACGTCAAAGGCCTAAGAA2000.25169263295663336No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCTTAGGTGAACCTGCGGAAGGATC1890.23784953814401855No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1840.23155722232010267No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC1790.22526490649618686No Hit
GGAAGTAAAAGTCGTAACAAGGTTCCAGACCGAAGAGATAGAGCTGCGAT1470.18499408522312552No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA1300.1636002114218117No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1280.16108328509224534No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1190.14975711660919686No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1140.143464800785281No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTATGTGAACCTGCGGAAGGATC1130.14220633762049786No Hit
GGAAGTAAAAGTCGTAACAAGGACCCTCCTTCCCCTGAGTCCTGCAGTTT1100.13843094812614834No Hit
GGAAGTAAAAGTCGTAACAAGCCAAAACCTGTGACATCAGTCATCGCTTT1060.13339709546701567No Hit
GGAAGTAAAAGTCGTAACAAGGCAGCCGTAGAAGTGAATGGTGTCTCCTA1060.13339709546701567No Hit
GGAAGTAAAAGTCGTAACAAGGTCTATTTTCTTTCATTGCACATAAAGCC1040.13088016913744935No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGTATC890.11200322166570184No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC800.10067705318265335No Hit
GGAAGTAAAAGTCGTAACTAAGAATGGGTTGCCTGCCAGCCCTGGCAGGT800.10067705318265335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)