FastQCFastQC Report
Sun 21 May 2023
EGAF00007832931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60280
Sequences flagged as poor quality0
Sequence length247-250
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1844230.59389515593895No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1607826.672196416721967No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA46797.762110152621101No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC46697.745520902455209No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC19383.214996682149967No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC16502.737226277372263No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC12072.002322495023225No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT6191.0268745852687458No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC5770.9571997345719974No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA5370.8908427339084273No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG4880.809555408095554No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTTAC4430.7349037823490379No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC4260.7067020570670206No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC4250.7050431320504313No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC3480.5773059057730591No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC2300.38155275381552756No Hit
GGAAGTAAAAGTCGTAACAAGGGTTATCAGGCCTTCTACAACAAGGAAAA2100.3483742534837425No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA1820.30192435301924353No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1670.27704047777040475No Hit
GGAAGTAAAAGTCGTAACAACTTCCAGGTTTCAGGTTGGAAAGTCCCTCA1590.2637690776376908No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA1430.23722627737226276No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1310.21731917717319177No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT1300.21566025215660253No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA1280.212342402123424No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCTTACGTGCA1210.20072992700729927No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA1160.192435301924353No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGAAGCAAAGGAAACACGGCTGTTTA1000.16589250165892502No Hit
GGAAGTAAAAGTCGTAACAAGGACAATTGATCGCACAAATTGATTCTGTA930.15428002654280026No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA880.145985401459854No Hit
GGAAGTAAAAGTCGTAACAAGGAGGCCTTGGCAGAAATGGAGAAAAATCT870.14432647644326477No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA840.13934970139349703No Hit
GGAAGTAAAAGTCGTAACAAGGCGTAGAGGAATTAGCGCAGAATCAGAAA840.13934970139349703No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTTATGGCTCAAGCAGAGCAAAA830.13769077637690777No Hit
GGAAGTAAAAGTCGTAACAAGGCTGTAGGTCCCGACCTCGCCGTTGATGA830.13769077637690777No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC750.12441937624419377No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC700.1161247511612475No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG650.10783012607830127No Hit
GGAAGTAAAAGTCGTAACAAGGTCGACAAGTTTATGGAGACCAGCGTCAA650.10783012607830127No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC630.10451227604512277No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGAATGAGGCTTTTGCCGAGTTTAA620.10285335102853352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)