FastQCFastQC Report
Sun 21 May 2023
EGAF00007832935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86456
Sequences flagged as poor quality0
Sequence length247-250
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4341050.21051170537615No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG53266.160359026556861No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC29643.428333487554363No Hit
GGAAGTAAAAGTCGTAACAAGGACAGGGCGGGTAACGAGGCCGGGGCGAG28013.2397982788933097No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT26833.1033126677153695No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA20062.320255390024984No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC17462.019524382344777No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA15731.819422596465254No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA15521.795132784306468No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA12911.4932451189044138No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC12631.4608587026926991No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAATCTATTATGGCAGGTGGAGGAGT9031.044461922827797No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC9011.0421486073841029No Hit
GGAAGTAAAAGTCGTAACAAGGACAGAGCAACGTATCAGGCGGCGGTGGC8110.9380494124178773No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT6250.7229110761543445No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG6070.7020912371610992No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCCATGGTTGTTG5330.6164985657444249No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC4050.4684463773480152No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC3560.4117701489775145No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC2780.3215508466734524No Hit
GGAAGTAAAAGTCGTAACAAGGGCGATGTGAAGACCAGATGCAATGCCAG2710.31345424262052374No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT2300.26603127602479876No Hit
GGAAGTAAAAGTCGTAACAAGGAGTTTAGGTCGGCACTAACTGCACTTGG2270.2625613028592579No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2250.260247987415564No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC2170.2509947256407884No Hit
GGAAGTAAAAGTCGTAACAAGGAGATGCAGAAAACGGCAGCGCTTCGGGA1980.2290182289256963No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1950.22554825576015544No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC1910.22092162487276765No Hit
GGAAGTAAAAGTCGTAACAAGGAATAGGACATGTCTCTAGCAGCCGTTAA1890.21860830942907378No Hit
GGAAGTAAAAGTCGTAACAAGGACAATTGATCGCACAAATTGATTCTGTA1830.21166836309799206No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC1730.20010178587952254No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC1730.20010178587952254No Hit
GGAAGTAAAAGTCGTAACAAGGAACGCAGATGTACTATGCCAAGAAGGGC1620.18737855093920605No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCTGAAGGATC1500.17349865827704264No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGTGAACCTGCGGTTGGATC1480.17118534283334874No Hit
GGAAGTAAAAGTCGTAACAAGGTATGAAGACTGCGACACTTTCAAAGCAT1400.16193208105857315No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG1370.1584621078930323No Hit
GGAAGTAAAAGTCGTAACAAGGATAGACTATGGAACAAACACCTAAAGCA1360.15730545017118536No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1360.15730545017118536No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCGATAGCTGCAGGTTTCCA1340.15499213472749143No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC1330.15383547700564448No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGTCTGGATC1320.15267881928379753No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCGATGGTAGTTG1290.1492088461182567No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1240.14342555750902194No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT1220.14111224206532802No Hit
GGAAGTAAAAGTCGTAACAAGGACACTTGGGACCGTAAGGATACCCGCAC1200.13879892662163412No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC1150.13301563801239938No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGAAGGGGAACCTGCGGAAGGATC1090.12607569168131766No Hit
GGAAGTAAAAGTCGTAACAAAGAGGATCTTAGCACATGCACATAGAAACA1030.11913574535023597No Hit
GGAAGTAAAAGTCGTAACAAGGTTAGTCGCACCGTAACTTCCGACAATCA1020.117979087628389No Hit
GGAAGTAAAAGTCGTAACAAGGCATGAGGTTGCAGGATATGGTGTTCGTA1020.117979087628389No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA1010.11682242990654204No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTATCCGGCA960.1110391412973073No Hit
GGAAGTAAAAGTCGTAACAAGGATAAAACCCAAAATAAGCCGGCAGTTGT950.10988248357546035No Hit
GGAAGTAAAAGTCGTAACAAGGTGGATGATGGTGCGTTGGTACTGGATAT940.10872582585361339No Hit
GGAAGTAAAAGTCGTAACAAGGATTTGCGCAGGTTGAAGGTGGTGTGCGC930.10756916813176645No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAATGATC910.10525585268807255No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAGAAGGACTACAGAAAGAAACAGGG910.10525585268807255No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGTATC900.10409919496622559No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGCAGCCATAGCAGTTGTTTGCGACG900.10409919496622559No Hit
GGAAGTAAAAGTCGTAACAACCAGAGATTCCAGGGGAAGCCCCATCTGGC880.1017858795225317No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCAGTAGGTGAACCTGCGGAAGGATC870.10062922180068473No Hit
GGAAGTAAAAGTCGTAACAAGGTTTACGTAGGTGAACCTGCGGAAGGATC870.10062922180068473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)