FastQCFastQC Report
Sun 21 May 2023
EGAF00007832936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86456
Sequences flagged as poor quality0
Sequence length235-238
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT2864133.127833811418526No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1055112.203895623207181No Hit
GCTGCGTTCTTCATCGATGCTACCCTTATCTGCAGCAGCTGGAATCTCTG53246.158045711113167No Hit
GCTGCGTTCTTCATCGATGCCTTCCTGGAGACCCCCTTCAGTGGGGATGG28953.3485241047469234No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT27753.209725178125289No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT24322.812991579531785No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC19192.219626168224299No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT16301.88535208661053No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT16221.8760988248357546No Hit
GCTGCGTTCTTCATCGATGCTGTGTTTAACAGCAAAGTGGAGAATAAGGA14951.729203294161192No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT13161.5221615619505877No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT11491.3289997224021468No Hit
GCTGCGTTCTTCATCGATGCCATTGAAAGTATACCAATAGCTTTCTCTAT9301.0756916813176645No Hit
GCTGCGTTCTTCATCGATGCCTGAACCAAGAGATCCATTGCTGAAAGTTT9111.0537151846025723No Hit
GCTGCGTTCTTCATCGATCTGAAAGAGCTCGGGATGCGCCCAGGCATCCG6780.7842139354122328No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG6150.7113444989358748No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCCGGGAAGGTCA4300.49736282039418894No Hit
GCTGCGTTCTTCATCGATGCAGCCCTTATCTGCAGCAGCTGGAATCTCTG4190.48463958545387253No Hit
GCTGCGTTCTTCATCGACTCGAAACGCCAAGGCATCCACCGTATGCCCTT4100.4742296659572499No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3870.44762653835477006No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGACGAGAGTCG3820.4418432497455353No Hit
GCTGCGTTCTTCATCGACACGAGAGCCAAGATATCCATTGCCAGGAGTCA3640.4210234107522902No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG3430.39673359859350416No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT3300.38169704820949385No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTA3210.3712871287128713No Hit
GCTGCGTTCTTCATCGATGCTATTGTCCACAGTGTCTGCTATCCAAAATA2840.3284907930045341No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG2820.3261774775608402No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG2450.283381141852503No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT2230.2579346719718701No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC2220.2567780142500231No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG2110.24405477930970668No Hit
GCTGCGTTCTTCATCGATGCGCCCTGTCCTCCCATCTGATAGAACTGCGC1980.2290182289256963No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT1960.22670491348200242No Hit
GCTGCGTTCTTCATCGATGCAATATCGGCTTGTGCTGTCTTAAACGCAGC1780.20588507448875726No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1760.2035717590450634No Hit
GCTGCGTTCTTCATCGATGCGAGAAACAAGAGATCCGTTGTTGAAAGTTT1750.2024151013232164No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG1740.20125844360136946No Hit
GCTGCGTTCTTCATCGAAACGTATATAGCTTCCGTCTTTTCTTCTTGTTT1670.19316183954844082No Hit
GCTGCGTTCTTCATCGATGCTCGAATTTTCGTGTGAGTTGCCGATATTGG1600.18506523549551215No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAAAACAGGAACAACACAATGTGTT1570.1815952623299713No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA1570.1815952623299713No Hit
GCTGCGTTCTTCATCGATGCTATTTAATTTTTCAAGAACGAGTTCATAAT1570.1815952623299713No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT1560.18043860460812436No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG1510.17465531599888962No Hit
GCTGCGTTCTTCATCGATGCGTGGTCATCATGTGGTGAGAAATCTTCTAA1490.17234200055519572No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCCAAAAGTTG1470.1700286851115018No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG1450.1677153696678079No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA1410.1630887387804201No Hit
GCTGCGTTCTTCATCGATCTGAAAGAGCTCGGGATGCTCCCAGGCATCCG1390.1607754233367262No Hit
GCTGCGTTCTTCATCGATAAAGAGTACACCACCAATCGCAGACTTAATGA1370.1584621078930323No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC1360.15730545017118536No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTTTTGAAAGTTT1300.15036550384010364No Hit
GCTGCGTTCTTCATCGATGCAGCCTTTATCTGCAGCAGCTGGAATCTCTG1270.1468955306745628No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTACCATCTTTTCTTCTGGTCT1260.14573887295271584No Hit
GCTGCGTTCTTCATCGATGCTCATATCCAGCAACCGCTGGGCGGAAACGC1250.1445822152308689No Hit
GCTGCGTTCTTCATCGATGCGAGATCCGAGATATCCGTTGCCGAGAGTCG1240.14342555750902194No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTA1220.14111224206532802No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC1210.13995558434348107No Hit
GCTGCGTTCTTCATCGATGCCGCCTTCCTTCACAATCGACCAGAAGTTCT1180.13648561117794022No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG1160.13417229573424633No Hit
GCTGCGTTCTTCATCGATGCGGTATCAGAAAGAATTTGGTAGGCCTGAGT1110.12838900712501156No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1080.12491903395947072No Hit
GCTGCGTTCTTCATCGATGGTTTGAACATACTTTTTGCACTTTGATAATT1060.1226057185157768No Hit
GCTGCGTTCTTCATCGATGTGAGAGCCGAGATATCCATTGCCGAGAGTCG1060.1226057185157768No Hit
GCTGCGTTCTTCATCGATACGAGAACCAAGAGATCCATTGTTAAAAGTTT1030.11913574535023597No Hit
GCTGCGTTCTTCATCGATGCCTTCCTTAACGTGGACAAAGGATTGGTCAA1020.117979087628389No Hit
GCTGCGTTCTTCATCGATGCGAGAGCTGAGATATCCGTTGCCGAGAGTCG1020.117979087628389No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCATTGCCGAGAGTCG980.11335245674100121No Hit
GCTGCGTTCTTCATCGATGCTTGGGAATAGGTTGCTGAACGAACGACTCT970.11219579901915425No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCTAGATATCCGTTGCCGGGAGTCG970.11219579901915425No Hit
GCTGCGTTCTTCATCGATGCACATTACGACAACAGCTGCACCGAAGCGCA950.10988248357546035No Hit
GCTGCGTTCTTCATCGATGCCCGCGAATCACGTAATACTCCGGCGGAACA930.10756916813176645No Hit
GCTGCGTTCTTCATCGATGCCTTCCTGTAGACCCCCTTCAGTGGGGATGG930.10756916813176645No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCGCCTGTCCGAGGT900.10409919496622559No Hit
GCTGCGTTCTTCATCGATGCCCTTGCCGAGCGCCACGGCGTTTCGTATTG900.10409919496622559No Hit
GCTGCGTTCTTCATCGATGCTGTCCGATGTTACGGTAGTGGGCGTCTTCA880.1017858795225317No Hit
GCTGCGTTCTTCATCGATGCCTTTCGCCGACAAACAACTAACGTAGATAT880.1017858795225317No Hit
GCTGCGTTCTTCATCGATGCCGACTTCAAACAGCAGTGGCATATCGGTGC870.10062922180068473No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC870.10062922180068473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)