FastQCFastQC Report
Sun 21 May 2023
EGAF00007832937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93027
Sequences flagged as poor quality0
Sequence length247-250
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC6171366.33880486310426No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC63816.859298913218743No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA38424.129983768153331No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT28843.1001752179474775No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGCGTGGTGTTCCAAGCAAGGCAGG13111.4092682769518527No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCGATGGTAGTTG10721.152353617766885No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC8730.9384372279015769No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC5910.6352994291979748No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACTGAATCCGAAAAGAGCACA4400.4729809625162587No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAGTGGAACTTTGCAGGGGATGAAAA4390.47190600578326725No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA3490.37515989981403247No Hit
GGAAGTAAAAGTCGTAACAAGGCAACGACGAGCACGATGCCGAGGATTTT3470.3730099863480495No Hit
GGAAGTAAAAGTCGTAACAAGGACAATTGATCGCACAAATTGATTCTGTA3270.35151085168821955No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACTTGCGGAAGGATC3090.3321616304943726No Hit
GGAAGTAAAAGTCGTAACAAGGTGCAGCAATGTTATAACCGCGATTGGAT3060.3289367602953981No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG2920.31388736603351713No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2890.31066249583454264No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC2750.2956131015726617No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC2630.28271362077676376No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC2300.24724004858804433No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC2180.23434056779214635No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG2120.2278908273941974No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC2010.2160663033312909No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT1980.2128414331323164No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC1810.19456716867146098No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1750.188117428273512No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1740.1870424715405205No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1730.185967514807529No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1680.18059273114257152No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC1610.17306803401163104No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC1590.17091812054564803No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTACGAGTGCTGGAAGG1460.15694368301675857No Hit
GGAAGTAAAAGTCGTAACAAGGCGATGAGGGGTTGGCCTTGATGCCCGCG1430.15371881281778407No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC1420.15264385608479258No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCTGAAGGATC1350.1451191589538521No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATA1310.14081933202188612No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA1170.12576993776000517No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTTGGTGAACCTGCGGAAGGATC1170.12576993776000517No Hit
GGAAGTAAAAGTCGTAACAAGGAACTGGCGACGTGCTTCAGGACTGCCTA1110.11932019736205618No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGTTGAACCTGCGGAAGGATC1060.1139454136970987No Hit
GGAAGTAAAAGTCGTAACAAGGTAGGAGAAGAAGAGCCCAGGCGAGGCAA1030.1107205434981242No Hit
GGAAGTAAAAGTCGTAACAAGGTCTTGAAAGCCGTTGAATACTGACATGA1030.1107205434981242No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGTAAGGATC1030.1107205434981242No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC1010.10857063003214121No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC1000.1074956732991497No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAGAAGGACTACAGAAAGAAACAGGG1000.1074956732991497No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCAGTAGGTGAACCTGCGGAAGGATC980.10534575983316673No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC970.10427080310017522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)