FastQCFastQC Report
Sun 21 May 2023
EGAF00007832947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190033
Sequences flagged as poor quality0
Sequence length247-250
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC7638340.19459778038551No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA7257338.189682844558575No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC138737.30031099861603No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC24621.2955644545947282No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG15720.8272247451758379No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC8820.46412991427804645No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCAGTGCTTG7660.4030878847358091No Hit
GGAAGTAAAAGTCGTAACAAGGTTCCAGACCGAAGAGATAGAGCTGCGAT7040.37046197239426837No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC6580.34625565033441563No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG5820.30626259649639803No Hit
GGAAGTAAAAGTCGTAACAAGGGGAAGTAGACCTGGCCCTCTTCCAGGGC5380.28310872322175623No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC5270.27732025490309575No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC4070.21417332779043638No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG3950.20785863507917046No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA3770.19838659601227157No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA3730.19628169844184956No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTACGGCA3640.1915456789084001No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCTTAGGTGAACCTGCGGAAGGATC3270.17207537638199683No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC3100.1631295617077034No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2960.15576242021122647No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT2880.15155262507038253No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA2840.14944772749996052No Hit
GGAAGTAAAAGTCGTAACAAGGTCTTCGTAGGTGAACCTGCGGAGGGATC2750.1447117079665111No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC2720.1431330347886946No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC2480.13050364936616274No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTATCCGGCA2470.12997742497355724No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC2410.12682007861792424No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC2360.12418895665489678No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA2300.12103161029926382No Hit
GGAAGTAAAAGTCGTAACAAGGTGTTGCCGTCTTTGTCGGTAACATCTTT2230.11734803955102534No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT2200.11576936637320887No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCAGGCA1930.10156130777286052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)