FastQCFastQC Report
Fri 2 Jun 2023
EGAF00007832949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74597
Sequences flagged as poor quality0
Sequence length247-250
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC3230543.30603107363567No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA1304117.481936270895613No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG43145.783074386369425No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA40695.454642948107833No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGTGTGGTGTTCCAAGCAAGGCAGG24893.3365953054412376No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGGTCAGCTAATCGACGCAATC17272.3151065056235502No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC15032.0148263334986662No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC12871.7252704532353849No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC11061.4826333498666167No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC6890.9236296365805595No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG6380.8552622759628403No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC6260.8391758381704358No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC6160.825770473343432No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA5860.7855543788624207No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC5220.6997600439695966No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT4490.6019008807324692No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG4350.5831333699746638No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA3170.4249500650160194No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC3060.4102041637063153No Hit
GGAAGTAAAAGTCGTAACAAGGACACTTGGGACCGTAAGGATACCCGCAC2810.37669075163880583No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAAGACAATAAAAAGACCTTCCGACG2380.31904768288268964No Hit
GGAAGTAAAAGTCGTAACAAGGAACTGGCAACGTGCTTCAGGACTGCCTA2340.31368553695188817No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT2340.31368553695188817No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT2290.30698285453838625No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC2150.288215343780581No Hit
GGAAGTAAAAGTCGTAACAAGGGGAGGTGTGGTGTTCCAAGCAAGGCAGG2110.28285319784977947No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCAATCGCAGCGGGTTTCCA1840.24665871281686932No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1370.1836534981299516No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG1260.16890759682024745No Hit
GGAAGTAAAAGTCGTAACAAGGGCACAGCATATCGGGGATTAGTCTATTC1250.1675670603375471No Hit
GGAAGTAAAAGTCGTAACAAGGAACTGAATGAGGCTTTTGCCGAGTTTAA1190.15952384144134482No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1180.15818330495864444No Hit
GGAAGTAAAAGTCGTAACAAGCCAAAACCTGTTACATCAGTCATCGCTTT1060.14209686716623993No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1020.13673472123543842No Hit
GGAAGTAAAAGTCGTAACAAGGAAATGGAGCAGGCCGTCCCCGAGGCGAA960.12869150233923615No Hit
GGAAGTAAAAGTCGTAACAAGGGCGATGTGAAGACCAGATGCAATGCCAG820.10992399158143089No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG780.10456184565062938No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG750.10054023620252825No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)