FastQCFastQC Report
Sun 21 May 2023
EGAF00007832977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60010
Sequences flagged as poor quality0
Sequence length247-250
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1979632.987835360773204No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1388623.139476753874355No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA611610.19163472754541No Hit
GGAAGTAAAAGTCGTAACAAGGTGGCTGAACTGACGTGGGAACAGGTCCG20643.439426762206299No Hit
GGAAGTAAAAGTCGTAACAAGGTACGGACGCATCGATGAAGAACGCAGCC12952.157973671054824No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC11871.9780036660556575No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC7241.2064655890684886No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC6761.1264789201799699No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC6201.0331611398100315No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG6011.0014997500416596No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT5940.9898350274954174No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCAAGGTACG4550.7582069655057491No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC3990.6648891851358107No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG3740.623229461756374No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT3620.6032327945342443No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC3170.5282452924512581No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC3070.5115814030994834No Hit
GGAAGTAAAAGTCGTAACAAGGATAATGCATTCAAGACATCCAGCCGATA2990.4982502916180636No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC2650.4415930678220297No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC2580.4299283452757874No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2320.38660223296117313No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC2110.35160806532244626No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC2050.3416097317113814No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1850.308281953007832No Hit
GGAAGTAAAAGTCGTAACAAGGCTATGTAAAAAATGACGGTAATAATGGT1840.30661556407265456No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT1720.2866188968505249No Hit
GGAAGTAAAAGTCGTAACAAGGTAAGCAAGTCAGGCAAAACCAAGTAACG1460.2432927845359107No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1420.23662722879520082No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT1270.21163139476753873No Hit
GGAAGTAAAAGTCGTAACAAGGTACGGACCATCGATGAAGAACGCAGCCA1160.19330111648058657No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA1020.16997167138810199No Hit
GGAAGTAAAAGTCGTAACAAGGACAATTGGAAGAAACCTGTTATGGCTGC970.16163972671221463No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC930.15497417097150473No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC870.14497583736043992No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGTGTGAACCCTGTTAAGCCTCTGCT820.13664389268455257No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC810.13497750374937512No Hit
GGAAGTAAAAGTCGTAACAAGGTCAAGCTCCTAAGGACAAAAAACAAGAT810.13497750374937512No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGTCGGTGAAAGGCCGGCGCTGTACC790.13164472587902015No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT750.12497917013831028No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAACCAACATGACTGATGAAGAAAAA740.12331278120313281No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGAGTGAGGAACGCAATCGTGAGAT730.12164639226795534No Hit
GGAAGTAAAAGTCGTAACAAGTCCGTAGTGGTGGCGCATGACGTGAAAGC690.11498083652724546No Hit
GGAAGTAAAAGTCGTAACAAGTTATGTAGATGAAGAACGCAGCCAAGGTA690.11498083652724546No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA670.11164805865689052No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA660.10998166972171304No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGAAACCTCTCCGGCA630.10498250291618064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)