FastQCFastQC Report
Sun 21 May 2023
EGAF00007832985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86844
Sequences flagged as poor quality0
Sequence length247-250
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC5770966.45133803141265No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG965011.111878771129842No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG44705.147160425590715No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC27143.125143936253512No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC8060.9281009626456634No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA8000.9211920224770853No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC7760.8935562618027727No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC6430.7404080880659574No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT5220.6010777946662982No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC3610.4156879001427848No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC3580.4122334300584957No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCGAT3430.39496107963705035No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC3020.34774998848509975No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC2940.33853806826032884No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG2810.32356869789507625No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC2730.3143567776703054No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT2500.2878725070240892No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC2500.2878725070240892No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC1850.213025655197826No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC1750.2015107549168624No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAAGATC1570.180783934411128No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGAGCAAAACCAGAAAACGCAATCAT1540.1773294643268389No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG1490.17157201418635715No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGATAGTCCTGAAGTATCTCTTCA1390.16005711390539357No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA1380.15890562387729723No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGCAGGTTCACCTACGGAAGGATC1120.12896688314679194No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1110.12781539311869558No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1100.12666390309059924No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC1100.12666390309059924No Hit
GGAAGTAAAAGTCGTAACAAGGGGAGCCTTGAATAAACAGCAAATTATTA1090.12551241306250288No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG970.11169453272534659No Hit
GGAAGTAAAAGTCGTAACAAGGTCCTGTGAGAGAAGGACAGAACTGAGGT870.10017963244438305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)