FastQCFastQC Report
Wed 8 May 2024
EGAF00007832999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007832999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174916
Total Bases43.5 Mbp
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC8330147.623430675295566No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA4173223.858309131240137No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC81774.6748153399345975No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC58683.3547531386494085No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG33601.9209220425804387No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGTTGCACAAACTCTTTTTTGATTT30531.7454092249994284No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA26171.496146721855062No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG19161.0953829266619406No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT16710.9553156943904504No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG14150.8089597292414645No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC13810.7895218276201148No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGCTGGATC13330.76208008415468No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC9600.5488348693086967No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG6690.38246929954949804No Hit
GGAAGTAAAAGTCGTAACAAGGGTGTAGAGTATAAGCTTGGAGCCCGTGG5930.3390198723958929No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC5780.3304443275629445No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC4760.2721306226988955No Hit
GGAAGTAAAAGTCGTAACAAGGTCGCAAAATTCAAAGATGAAATATACCA4720.26984381074344255No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC4440.25383612705527225No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA3980.22753778956756385No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGATGAAAATGACAAGAAAAGCGACA3110.1777996295364632No Hit
GGACGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC2940.16808067872578838No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA2920.16693727274806192No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGACCCTGCGGCTGGATC2860.1635070548148826No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGTGGCTGGAAC2430.13892382629376385No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC2360.13492190537172127No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACTGAATCCGAAAAGAGCACA2180.12463125157218323No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA1970.11262548880605547No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGCAGTTGTTTGGATACGCCGCGCGT1840.10519334995083354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)