FastQCFastQC Report
Sun 21 May 2023
EGAF00007833019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422278
Sequences flagged as poor quality0
Sequence length247-250
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC15197735.98979818981808No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC279106.609390022686476No Hit
GGAAGTAAAAGTCGTAACAAGGGCGGCGAAGACGTCGAGGAAGAAGCGGC230005.446648890067681No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC194024.594603555004049No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA188914.473593225316024No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC171324.0570429906365No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC138113.2705942530749885No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTACGAGTGCTGGAAGG129563.0681210008572553No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG103462.450044757245227No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA81271.9245615447643496No Hit
GGAAGTAAAAGTCGTAACAAGGATGTGCATGTACCCATCGTAAAGCGAAG49561.1736344303989314No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAGGAAGCTGG45741.0831726966595465No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC40130.9503218259061567No Hit
GGAAGTAAAAGTCGTAACAAGGGCAATAAGAAGAAGGAGAAGGGCGTCGA38240.9055645806790786No Hit
GGAAGTAAAAGTCGTAACAAGGGTTGATGAGACAACTTACACATTTGTAA37790.8949080937202506No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA36620.8672012276272977No Hit
GGAAGTAAAAGTCGTAACAAGGCCGTGAAGTAAGCGGGAAGCATATTGAT31180.7383761408361316No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC29700.7033281392826526No Hit
GGAAGTAAAAGTCGTAACAAGGATGTGCATGTACCCATCGTAAAGCGAAA21890.5183788878416589No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT20470.4847517512160236No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG20180.47788423739811214No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC20150.4771738049341903No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC19860.47030629111627886No Hit
GGAAGTAAAAGTCGTAACAAGGCAACGACGAGCACGATGCCGAGGATTTT17360.41110358578945627No Hit
GGAAGTAAAAGTCGTAACAAAGGTTGATGAGACAACTTACACATTTGTAA16890.39997347718801357No Hit
GGAAGTAAAAGTCGTAACAAGGGTGTAGAGTATAAGCTTGGAGCCCGTGG16730.396184504047097No Hit
GGAAGTAAAAGTCGTAACAAGGAAGCGCAGGTGCTGCAGGACGGTCGTCA16660.39452682829794594No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCATAGGTGAACCTGCGGAAGGATC15260.3613733133149252No Hit
GGAAGTAAAAGTCGTAACAAGGTCCTGTGAGAGAAGGACAGAACTGAGGT15220.36042607002969607No Hit
GGAAGTAAAAGTCGTAACAAGGTGCGAGACCTCGAAGACCTGATGGAAAA14800.35048001553478986No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA14750.3492959614282534No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAAAGGACCGGAATCTGCTTCATACG13660.32348358190575877No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG13070.30951174344862864No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC13050.30903812180601403No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC12840.30406509455856096No Hit
GGAAGTAAAAGTCGTAACAAGGTTGATGGTAGTAAATGAATTATTACCAC12430.294355850884962No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCGATGGTAGTTG11690.27683185010822253No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC11590.27446374189514966No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA11460.2713852012181549No Hit
GGAAGTAAAAGTCGTAACAAGGGAAAGAAGGGGCTTACGGAGATGGATAA9920.23491633473683213No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT9140.2164450906748635No Hit
GGAAGTAAAAGTCGTAACAAGGCGGCAGCCGCAGTGGAATCCGTCGTGCG8940.21170887424871768No Hit
GGAAGTAAAAGTCGTAACAAGGCGGGCACAAAGGCGATCGTGTAGTATCC8890.2105248201421812No Hit
GGAAGTAAAAGTCGTAACAAGGACGCACATCATGATGCTCAAGAAGCTGG8750.20720946864387918No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC8690.20578860371603538No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC7870.1863701163688376No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC7490.17737130515916055No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC7440.1761872510526241No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCAGAAGGATC6740.15961049356111376No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGTC6710.1589000610971919No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGCGTGGTGTTCCAAGCAAGGCAGG6550.15511108795627526No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT6390.15132211481535862No Hit
GGAAGTAAAAGTCGTAACAAGGGACGTACCGGCTCTGTAGCTGCTTACTA6230.14753314167444195No Hit
GGAAGTAAAAGTCGTAACAAGGGAGAACAGAAGAATGGAAAGAAATCTGA5680.134508546502541No Hit
GGAAGTAAAAGTCGTAACAAGGCAATGAACGAAGTGAATCGGAAAGAGAA5600.13261405993208267No Hit
GGAAGTAAAAGTCGTAACAAGGTTGATGAGACAACTTACACATTTGTAAT5430.12858827596985872No Hit
GGAAGTAAAAGTCGTAACAAGGTCGATAAGACCCCAGAAGTTGGACTCAG5160.12219438379456188No Hit
GGAAGTAAAAGTCGTAACAAAGTTTTGGTAGGTGAACCTGTGGAGGGATC5080.12029989722410356No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTAAGGTATC4940.1169845457258015No Hit
GGAAGTAAAAGTCGTAACAAGGTAGGAGAAGAAGAGCCCAGGCGAGGCAA4880.11556368079795774No Hit
GGAAGTAAAAGTCGTAACAAGGAGGACAAGAAGGAGCAGGAAAAGGATAA4820.114142815870114No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGTGAACCTGCGGTTGGATC4690.11106427519311922No Hit
GGAAGTAAAAGTCGTAACAAGGATAGGTGAAATCTCAGAGAAAGCGTAGA4630.10964341026527549No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC4440.10514400466043697No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC4370.10348632891128594No Hit
GGAAGTAAAAGTCGTAACAAGGGAGAAAACGGTGAATGTGAGCGAAGAAG4340.10277589644736407No Hit
GGAAGTAAAAGTCGTAACAAGGCGATGAGGGGTTGGCCTTGATGCCCGCG4240.10040778823429115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)