FastQCFastQC Report
Sun 21 May 2023
EGAF00007833022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66230
Sequences flagged as poor quality0
Sequence length235-238
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1311119.79616487996376No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT832112.563792843122451No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCGCCTGTCCGAGGT62589.448890231013136No Hit
GCTGCGTTCTTCATCGATGCTACCCTTATCTGCAGCAGCTGGAATCTCTG51517.7774422467159905No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG38925.87649101615582No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT25193.8034123508983844No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG22653.4199003472746488No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT17672.66797523780764No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTACCATCTTTTCTTCTGGTCT14602.2044390759474557No Hit
GCTGCGTTCTTCATCGATGCTGCCACCACGTTTAACATCATGCGTTTTGG11771.7771402687603806No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC9601.4494941869243545No Hit
GCTGCGTTCTTCATCGATGCGTTGATATTCCACTTCGCAAATCAAAATAA8381.265287634002718No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG6681.0086063717348632No Hit
GCTGCGTTCTTCATCGATGCCGCAATGGTAGGGCATTTAGGCTCAGCAGC6530.9859580250641703No Hit
GCTGCGTTCTTCATCGATGCCCTCTATCAGACGAATGGTAAAGGGGATAT5310.8017514721425336No Hit
GCTGCGTTCTTCATCGATGCGGATACGCTGAGCACCGTGACGCCGGACAA5080.767024007247471No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT4880.7368262116865469No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT4830.7292767627963158No Hit
GCTGCGTTCTTCATCGATGCTCACTGACGATCCCTACCACCTCGCTCCCT4250.6417031556696361No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT4180.6311339272233126No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT4070.6145251396648044No Hit
GCTGCGTTCTTCATCGATGCAATGAGCGGAGAAACGACAATCGCCGTTCC3260.49222406764306204No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3160.47712516986260006No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG2930.4423977049675374No Hit
GCTGCGTTCTTCATCGATGCTGTGGTGGTAACGGTGGCCGCGGCGCTGGG2700.40767024007247477No Hit
GCTGCGTTCTTCATCGATGAAGTCGAGAGAAATTTGCGCCTGTCCGAGGT2480.37445266495545826No Hit
GCTGCGTTCTTCATCGATGCATAGCTTAATTGCATCTGTAGGTCTCCTAT2430.36690321606522724No Hit
GCTGCGTTCTTCATCGATGCGGGGCAAGTAGACCAGTTACAAGCGGGCCC2250.3397252000603956No Hit
GCTGCGTTCTTCATCGATGCCGAGGAGGACACGTACGTCGCGCACTGCGG1940.29291861694096333No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG1920.2898988373848709No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT1850.27932960893854747No Hit
GCTGCGTTCTTCATCGATGGTCGCGCGGCACTCGACGTGCACACGGCGGA1830.2763098293824551No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA1710.25819115204590065No Hit
GCTGCGTTCTTCATCGACTCCCAGTGCCAAGGCATCCGCCTTATGCTCTT1620.24460214404348485No Hit
GCTGCGTTCTTCATCGACTCCTGACGCCAAGGCATCCACCGTATGCCTTT1470.22195379737279178No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG1380.20836478937037595No Hit
GCTGCGTTCTTCATCGATGCCATCCGCGACGAATAAGTCCACTGCTGCCG1370.20685489959232978No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT1260.19024611203382152No Hit
GCTGCGTTCTTCATCGATGCACTGATCTCGCAGATGAGCGAGCACCCCAT1220.18420655292163673No Hit
GCTGCGTTCTTCATCGATGCCGCTGCGGAGGAAGCACTGCCGCTTGAGAA1130.1706175449192209No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT1100.16608787558508228No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC1000.15098897780462028No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT970.14645930847048166No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT960.14494941869243544No Hit
GCTGCGTTCTTCATCGACACGAGAGCCAAGATATCCATTGCCAGGAGTCA950.14343952891438924No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT930.14041974935829685No Hit
GCTGCGTTCTTCATCGATGCGCCCCCAGGCCTGTTTGACGATCAGGCCGT920.13890985958025065No Hit
GCTGCGTTCTTCATCGATGCGTTGATATTTCACTTCGCAAATCAAAATAA850.1283406311339272No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTCGCAAATCA850.1283406311339272No Hit
GCTGCGTTCTTCATCGATGCGGGGCAAGTAGACCAGCTACAAGCGGGTCC840.12683074135588102No Hit
GCTGCGTTCTTCATCGATGCAAATCCGCCGATTCCAAGCTCCGAGATAGC830.12532085157783482No Hit
GCTGCGTTCTTCATCGATGCCGGTCGCTTTGGAAACCGCCGTGCGGACAT810.12230107202174242No Hit
GCTGCGTTCTTCATCGATGCTTTTTGGCGCAACGGCGACATTGTCGCGTT790.11928129246565002No Hit
GCTGCGTTCTTCATCGATGCCGCGTACTGTTCCGCAAGCGCGGGCGCCGG780.11777140268760382No Hit
GCTGCGTTCTTCATCGATGCTTGGGAATAGGTTGCTGAACGAACGACTTT710.10720217424128038No Hit
GCTGCGTTCTTCATCGATGCGTCGGACAGTCACAATCAACTCACATGCGG700.10569228446323418No Hit
GCTGCGTTCTTCATCGATGCCGAAGACCACGACCTCGGACTAAGACGCCT700.10569228446323418No Hit
GCTGCGTTCTTCATCGATGCTAGAATTCTCGTGAGAGTTACCAATATTGG680.10267250490714178No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)