FastQCFastQC Report
Sun 21 May 2023
EGAF00007833035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70153
Sequences flagged as poor quality0
Sequence length247-250
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1646523.470129573931263No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1553222.14017932233832No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1433320.43105783074138No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC49177.008966116915884No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC42316.031103445326643No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC21012.9948826137157356No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG19212.7383005716077715No Hit
GGAAGTAAAAGTCGTAACAAGGAACGTTCAGCGATAGCTGCAGGTTTCCA5930.8452952831667925No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG5550.7911279631662225No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC4050.5773095947429191No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC3990.5687568600059869No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC3470.4946331589525751No Hit
GGAAGTAAAAGTCGTAACAAGGTTCCAGACCGAAGAGATAAAGCTGCGAT3000.42763673684660675No Hit
GGAAGTAAAAGTCGTAACAAGGAACTGTTGAAAAAGCTGGTAATTGTCAT2970.42336036947814065No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC2590.3691930494775705No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGTGGAAGGATC1710.24375294000256584No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA1590.22664747052870154No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATC1530.2180947357917694No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGCTGGATC1490.21239291263381466No Hit
GGAAGTAAAAGTCGTAACAAGGAATTGTTGAAAAAGCTGGTAATTGTCAT1320.18816016421250695No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC1290.18388379684404088No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGCAATCGGGATTTTAGCCGGTGAA1280.1824583410545522No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC1270.1810328852650635No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG1220.17390560631762006No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA1090.1553746810542671No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGTTGCGGCTGGATC1020.14539649052784628No Hit
GGAAGTAAAAGTCGTAACAAGGCCGCTGGACAACAACAGGCGGCACACCA1000.1425455789488689No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACATCTCTCCGGCA970.13826921158040284No Hit
GGAAGTAAAAGTCGTAACAAGGCGATCGGACAGTTTGACCTGTTCGGTGC910.1297164768434707No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA840.11973828631704987No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTACGGCA810.1154619189485838No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGAAACCTCTCCGGCA730.10405827263267431No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)