FastQCFastQC Report
Sun 21 May 2023
EGAF00007833039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59922
Sequences flagged as poor quality0
Sequence length247-250
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC3981366.4413737859217No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA33055.515503487867561No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC15572.5983778912586364No Hit
GGAAGTAAAAGTCGTAACAAGGACCGGGCAATAAGCGTGGCGGAAAGAAC8891.4835953406094589No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC8331.3901405160041387No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC8081.3484196121624779No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA7331.2232569006374954No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA6261.0446914321951872No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAATGATC4890.8160608791428858No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGGCGAGGAAAATGGTGAGGGAGA4870.812723206835553No Hit
GGAAGTAAAAGTCGTAACAAGGAAGACAACTGAGGAATGGTGATGTTGCG4690.7826841560695571No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT3910.6525149360835752No Hit
GGAAGTAAAAGTCGTAACAGGCGTTAAGTTTACAGCATGAAGAGAACAAA3680.6141317045492474No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC3250.5423717499415908No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC2930.48896899302426483No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG2450.4088648576482761No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC2220.37048162611394814No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGTAAGGATC2150.3587997730382831No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGATCAGAGAAAGGCGATCAGAG2080.34711791996261804No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTATGTGAACCTGCGGAAGGATC2060.3437802476552852No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2030.3387737391942859No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1630.2720202930476286No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAATCTATTATGGCAGGTGGAGGAGT1580.26367611227929644No Hit
GGAAGTAAAAGTCGTAACAAGGAGAAATGATGTCGATCAAGAAGATTGCG1480.24698775074263207No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1480.24698775074263207No Hit
GGAAGTAAAAGTCGTAACAACAGGAGAGCGGTCAGCTAATCGACGCAATC1420.2369747338206335No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT1380.23029938920596776No Hit
GGAAGTAAAAGTCGTAACAAGGTTTACGTAGGTGAACCTGCGGAAGGATC1380.23029938920596776No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1320.22028637228396916No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAACACA1250.2086045192083041No Hit
GGAAGTAAAAGTCGTAACAAAATGCATCGTACAGCAGGCCAAGTGGTTGT1220.20359801074730485No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1210.2019291745936384No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1140.19024732151797336No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAAGACAATAAAAAGACCTTCCGACG1100.18357197690330762No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1080.18023430459597475No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGTATC1070.17856546844230833No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC1000.1668836153666433No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACATGCGGAAGGATC950.15853943459831116No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGAAGGTGAACCTGCGGAAGGATC880.1468575815226461No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC880.1468575815226461No Hit
GGAAGTAAAAGTCGTAACAAGACTACGCTCTGTGACGTTGCTCTGACGGA840.14018223690798037No Hit
GGAAGTAAAAGTCGTAACAAGGATGGCATCATTGGTTACGTTCTTGCAAT820.1368445646006475No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAGAAGGACTACAGAAAGAAACAGGG820.1368445646006475No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTAGGTGAACCTGCGGAAGGATC790.13183805613964822No Hit
GGAAGTAAAAGTCGTAACAAGGGTGAGCAGAAAGGCTTTGAAAGAGTAAG790.13183805613964822No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCTGAAGGATC770.12850038383231532No Hit
GGAAGTAAAAGTCGTAACAAGGCGATGTGGAAACAGGACTCGTATTCACG690.11514969460298388No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC630.10513667768098528No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTTAACCTGCGGAAGGATC630.10513667768098528No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC620.10346784152731885No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGAGAACCTGCGGAAGGATC610.10179900537365243No Hit
GGAAGTAAAAGTCGTAACAACCGACCAAGTGGCTGACCGATTGGCATTGA600.10013016921998598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)