FastQCFastQC Report
Sun 21 May 2023
EGAF00007833040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59922
Sequences flagged as poor quality0
Sequence length237-238
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2056434.31794666399653No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT720012.015620306398318No Hit
GCTGCGTTCTTCATCGACACGAGAGCCAAGATATCCATTGCCAGGAGTCA38326.394980140849771No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT37736.296518807783452No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT21103.521244284236174No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGGGATATCCGTTGCCGAGAGTCA18803.137411968892894No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT14702.453189145889657No Hit
GCTGCGTTCTTCATCGACTCGAAACGCCAAGGCATCCACCGTATGCCCTT11821.9725643336337237No Hit
GCTGCGTTCTTCATCGATGCCAGCTCGCTTACAGTTACAAATTCGGTAAG8231.3734521544674745No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG8051.3434131037014785No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT7711.2866726744768198No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG6291.0496979406561864No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGGT5600.9345482460532024No Hit
GCTGCGTTCTTCATCGATGCCTTGCAGTGGGTCTTCATAGTGCCTATCCA4940.8244050599112179No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG4070.6792163145422383No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC3990.6658656253129068No Hit
GCTGCGTTCTTCATCGATGCAGTAAGACCGTAGCTGCGTTCTTCATCGAT3680.6141317045492474No Hit
GCTGCGTTCTTCATCGATGCAATAGCCGAGATATCCGTTGCCGAGAGTCG2950.4923066653315977No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT2940.4906378291779313No Hit
GCTGCGTTCTTCATCGATGAGAGAGCCGAGATATCCGTTGCCGAGAGTCG2910.485631320716932No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAATTTT2900.48396248456326557No Hit
GCTGCGTTCTTCATCGATGCTAGAGCCGAGATATCCGTTGCCGAGAGTCG2580.43055972764593975No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG2580.43055972764593975No Hit
GCTGCGTTCTTCATCGATGCCTTCCTGAACGTGGACAAAGGATTGGTCAA2550.4255532191849405No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGACGAGAGTCG2540.42388438303127396No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC2500.4172090384166082No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG2470.41220252995560897No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT2460.41053369380194255No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTAG2360.3938453322652782No Hit
GCTGCGTTCTTCATCGATGCCTTCCTTAACGTGGACAAAGGATTGGTCAA2180.3638062814992824No Hit
GCTGCGTTCTTCATCGATGCAGGAACCAAGAGATCCATTGTTGAAAGTTT2030.3387737391942859No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCAGAGAGTCG1750.29204632689162574No Hit
GCTGCGTTCTTCATCGATGCGATAGCCGAGATATCCGTTGCCGAGAGTCG1730.2887086545842929No Hit
GCTGCGTTCTTCATCGATGCCATTGAAAGTATACCAATAGCTTTCTCTAT1640.273689129201295No Hit
GCTGCGTTCTTCATCGATGCGAGATCCGGGATATCCGTTGCCGAGAGTCA1630.2720202930476286No Hit
GCTGCGTTCTTCATCGATGCGGTACCGACTTCGAAGACTTGCAGGATGGC1550.2586696038182971No Hit
GCTGCGTTCTTCATCGATGCTGTGGTGGTGACGGTGGCTGCTGCGCTGGG1520.2536630953572978No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG1500.25032542304996497No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTACCATCTTTTCTTCTGGTCT1440.24031240612796637No Hit
GCTGCGTTCTTCATCGACTCCTGACGCCAAGGCATCCACCGTATGCCTTT1390.23196822535963418No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCCGGGAAAGTCA1320.22028637228396916No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT1280.21361102766930345No Hit
GCTGCGTTCTTCATCGATGCTTTTTCTTGTCAGAGATTACGGGGCTCTGT1210.2019291745936384No Hit
GCTGCGTTCTTCATCGATGCTGGAGCCAAGAGATCCGTTGTTGAAAGTTT1200.20026033843997196No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT1150.1919161576716398No Hit
GCTGCGTTCTTCATCGATGTTTTGCAGTTTGCTCGTCTGACTGAAACGGA1140.19024732151797336No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT1080.18023430459597475No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGGCGAGAGTCG1060.17689663228864191No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGATATATCCGTTGCCGAGAGTCG1050.17522779613497547No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG980.16354594305931042No Hit
GCTGCGTTCTTCATCGATGCAAGATCCGAGATATCCGTTGCCGAGAGTCG910.1518640899836454No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCGGTTGCCGAGAGTCG900.15019525382997898No Hit
GCTGCGTTCTTCATCGATACGAGAGCCAAGATATCCATTGCCAGGAGTCA850.14185107306164682No Hit
GCTGCGTTCTTCATCGATGAAAGAGCCGAGATATCCGTTGCCGAGAGTCG840.14018223690798037No Hit
GCTGCGTTCTTCATCGATGCGCCCGACAAGGATGCCATTGAGAAGAATAT830.13851340075431395No Hit
GCTGCGTTCTTCATCGATGCAAATCCTCCGATTCCGAGCTCGGAAATGGC820.1368445646006475No Hit
GCTGCGTTCTTCATCGATGCTAGGCTTTTTACTTCTTCTTTTGCTTCAGC820.1368445646006475No Hit
GCTGCGTTCTTCATCGCTGTCAGATGCCTTGGCATCCTTCGTATGCCCTT810.13517572844698109No Hit
GCTGCGTTCTTCATCGGTGAACGCTGCACGTGGTGTTCTCGATAGAACGA790.13183805613964822No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTTTTGAAAGTTT770.12850038383231532No Hit
GCTGCGTTCTTCATCGATGCCAGTACCAAGTGCCTTACGGACACCACGAG770.12850038383231532No Hit
GCTGCGTTCTTCATCGATGCAGTAAGACCGTAGCTGCGTTCTTCATCGGC760.1268315476786489No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCTTTGCCGAGATTCG750.12516271152498248No Hit
GCTGCGTTCTTCATCGATGCGAGAGCAGAGATATCCGTTGCCGAGAGTCG730.1218250392176496No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAATTTT730.1218250392176496No Hit
GCTGCGTTCTTCATCGATGCCATCTCGCTTACAGTTACAAATTCGGTAAG680.11348085844931745No Hit
GCTGCGTTCTTCATCGATGCCGTTCTTTCCTGCAAGAAGCGCCTGCCAGA680.11348085844931745No Hit
GCTGCGTTCTTCATCGATGAACGCTGCACGTGGTGTTCTCGATAGAACGA670.111812022295651No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT670.111812022295651No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGACGAGAGTCG650.10847434998831815No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)