FastQCFastQC Report
Sun 21 May 2023
EGAF00007833052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78195
Sequences flagged as poor quality0
Sequence length235-238
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT1709221.858175075132678No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1354017.315685146109086No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1294516.554766928831764No Hit
GCTGCGTTCTTCATCGATGCTCCATGTTATATGGATTTCATAACTAATTC53896.891744996483151No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG41035.247138563846794No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG30053.842956710787135No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT17732.267408402071744No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA15001.918281220026856No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT13981.7878380970650298No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT12891.6484429950764115No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG12411.587057996035552No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG7260.9284481104929982No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA6740.8619476948654006No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT6460.8261397787582326No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT5130.6560521772491847No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT4870.6228019694353859No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT4100.5243302001406739No Hit
GCTGCGTTCTTCATCGATGCCATATATTTGCTTATCACAGCCAGCACTGG3470.44376238889954606No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAAGGGTTCGATGCGCGT2660.3401752030180958No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT2630.3363386405780421No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT2570.3286655156979347No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT2300.2941364537374513No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAATCACAGCCAAGTAGCAG2100.2685593708037598No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG2100.2685593708037598No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG2070.2647228083637061No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT2030.25960739177696784No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGTCCGGACGATCGGGCAAACT2000.25577082933691414No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGATAGTCG1970.2519342668968604No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC1820.23275145469659184No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT1640.2097320800562696No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG1520.19438583029605475No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT1480.18927041370931647No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGTCAAACT1460.1867127054159473No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACTATCGGGCAAACT1450.18543385126926273No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG1440.18415499712257816No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACTAAGCACAGCCAAGTAGCAG1400.17903958053583988No Hit
GCTGCGTTCTTCATCGATGCTCCATGTTATATGGATTTCATAACTAATAC1310.16752989321567874No Hit
GCTGCGTTCTTCATCGATGCCTGCGGATGCAATTGCCTCGACACTCGGGT1290.1649721849223096No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGATGCCGGACGATCGGGCAAACT1200.15346249760214847No Hit
GCTGCGTTCTTCATCGATGCCAGTTGGCGGGTGAACACTGTAATGTCTTC1150.14706822686872564No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTTG1110.14195281028198733No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCTAGGCCGGACGATCGGGCAAACT1100.14067395613530276No Hit
GCTGCGTTCTTCATCGACTCGAAACGCCTAGGCATCCACCGTATGCCCTT980.12532770637508792No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG950.12149114393503421No Hit
GCTGCGTTCTTCATCGACACGAGAGCCAAGATATCCATTGCCAGGAGTCA920.11765458149498048No Hit
GCTGCGTTCTTCATCGATGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA890.11381801905492679No Hit
GCTGCGTTCTTCATCGATGGGAGAACCTAGAGATCCGTTGTCGAAAGTTG870.11126031076155764No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG840.10742374832150393No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATACGTTGCCGAGAGTCG830.10614489417481936No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGTGCAAACT830.10614489417481936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)