FastQCFastQC Report
Sun 21 May 2023
EGAF00007833071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86479
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4367750.50590316724291No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA64737.485054175002023No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG63847.382139016408608No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT59186.84327987141387No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC21792.5196868603938527No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA18832.1774072318135036No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG17582.0328634697440995No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC12151.404965367314608No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCAGGGCTTCGACCTTCATGCCGCGT12021.3899328160593902No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG9201.0638420888308144No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC7910.9146729263751893No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA6900.7978815666231108No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC6730.7782236149816718No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGAGAAACACGTCAAAGGCCTAAGAA5440.6290544525260469No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG4490.5192011933532996No Hit
GGAAGTAAAAGTCGTAACAAGGACAGTAAAGTCATCCCGACCCAGCGCGA3360.38853363244255834No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC3200.37003203089767456No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC3060.3538431295459013No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2710.3133708761664682No Hit
GGAAGTAAAAGTCGTAACAAGGGGCTCGGGAAAAAGGCAAAGAGAAACAT2670.3087454757802472No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2670.3087454757802472No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC2630.3041200753940263No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGATCCAGTCGAGCATCTCTCGACG2200.25439702124215124No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC2170.2509279709524856No Hit
GGAAGTAAAAGTCGTAACTAAGAATGGGTTGCCTGCCAGCCCTGGCAGGT2040.23589541969726754No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG1980.22895731911793615No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAGGTAAGGGTTTGGTGGTATTGGTC1890.21855016824893903No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC1670.1931104661247239No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAAACTGCGGAAGGATC1550.17923426496606112No Hit
GGAAGTAAAAGTCGTAACAAGGTGGGCTGCATGCATCTCACTTCCATGCC1380.15957631332462216No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGAGGAAGGATC1380.15957631332462216No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC1360.15726361313151171No Hit
GGAAGTAAAAGTCGTAACAAGGTTTGAGGGAGGCACATCTCACGCATGCG1360.15726361313151171No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAATAAGGACAGATCCATTAGGAGGC1300.1503255125521803No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGCAGTCGGGGTACCAGTGCCCAGA1210.1399183616831832No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTCAC1160.13413661120040704No Hit
GGAAGTAAAAGTCGTAACAAGGTGGAGGGTAGCTCCTTGCGAAACCTACC1160.13413661120040704No Hit
GGAAGTAAAAGTCGTAACAAGGATGGAAAGAGGTGGGGGAGGAGAAGAGG1120.1295112108141861No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA1120.1295112108141861No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1090.1260421605245204No Hit
GGAAGTAAAAGTCGTAACAAGGGATGACAGGGAAGGAGGAAGTACAGGGG1080.12488581042796518No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1060.1225731102348547No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGGTGGCGTCTCTGAGGGCAAAGCT1040.12026041004174423No Hit
GGAAGTAAAAGTCGTAACAAAGTTTTGGTAGGTGAACCTGTGGAGGGATC1030.119104059945189No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAAAGGACCGGAATCTGCTTCATACG1020.11794770984863377No Hit
GGAAGTAAAAGTCGTAACAAGGAAGATATGAAAAGGGGCCAAAACCAAGG1020.11794770984863377No Hit
GGAAGTAAAAGTCGTAACAAGGATGCGACTGAATAACGTGGTCGATTAAC1010.11679135975207855No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA970.1121659593658576No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATTGGCTTGAGGGGGTGGGCAGT910.10522785878652621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)