FastQCFastQC Report
Sun 21 May 2023
EGAF00007833072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86479
Sequences flagged as poor quality0
Sequence length235-238
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT2153424.90084297922039No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1654919.13643774789255No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG64577.46655257345714No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT60036.941569629621064No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC56466.5287526451508455No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT23082.6688560228494778No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT17181.9866094658818902No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT15871.835127603233155No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT13841.6003885336324426No Hit
GCTGCGTTCTTCATCGATGCCCAGTGAAGTGCAGCGGGCAGCATCGGTCA12551.4512193711768175No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT10681.2349819031209888No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC9191.0626857387342592No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT7230.8360411198094334No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT7180.8302593693266573No Hit
GCTGCGTTCTTCATCGATGCCGGAGCCAAGAGATCCGTTGTTGAAAGTTT6610.7643474138230091No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA6510.7527839128574567No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG5940.6868719573538085No Hit
GCTGCGTTCTTCATCGATGCACGAGCCGAGTGATCCACCGCTAAGAGTCG5850.6764648064848113No Hit
GCTGCGTTCTTCATCGATGCAAACCTCGGCCGCACCCCAAGGCCTTGCTG5360.619803651753605No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTAAAAGTTT4820.5573607465396224No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG4500.5203575434498549No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT3230.37350108118734027No Hit
GCTGCGTTCTTCATCGATGCCGATATGTTTCTCAAAATCACCCTGACCAT3050.3526867794493461No Hit
GCTGCGTTCTTCATCGATGCGGTTGATGGTGGTGCCGCCGTAAATATGGC2980.34459232877345947No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG2760.3191526266492443No Hit
GCTGCGTTCTTCATCGATGCGATAGAGGACCTGATCCTTGGTGACATGAT2610.30180737520091583No Hit
GCTGCGTTCTTCATCGATGCCTATCACACCATTTAAGATGTTTTCCTGTG2610.30180737520091583No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT2530.29255657442847394No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2480.2867748239456978No Hit
GCTGCGTTCTTCATCGATGCCACCACCTCAATGCCATCTCGCTGATCTGG2020.2335827195041571No Hit
GCTGCGTTCTTCATCGATGCCGAGCCAGAAGTGATGGTTTTAGTGGGGAA1980.22895731911793615No Hit
GCTGCGTTCTTCATCGAGGTAGTGTCGTGCTTCGGGAGGTTTTCCCACAC1970.2278009690213809No Hit
GCTGCGTTCTTCATCGATGCTCCATGTTATATGGATTTCATAACTAATAC1780.20583031718683148No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG1580.18270331525572683No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT1550.17923426496606112No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG1550.17923426496606112No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT1470.16998346419361926No Hit
GCTGCGTTCTTCATCGATCACACCTGCCATCCCCTGCCGGGCCCAACCTC1460.16882711409706402No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG1430.16535806380739834No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1290.1491691624556251No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCTGAGAGTCG1270.1468564622625146No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA1270.1468564622625146No Hit
GCTGCGTTCTTCATCGATACGAGAACCAAGAGATCCATTGTTAAAAGTTT1220.14107471177973843No Hit
GCTGCGTTCTTCATCGATGCGCACACTACTCCCACCGCCCCCGAGTGCCT1220.14107471177973843No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA1210.1399183616831832No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT1200.13876201158662796No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT1160.13413661120040704No Hit
GCTGCGTTCTTCATCGATGCCATCTTGCCATGGTTGAGGAGGGTGGGAGC1150.1329802611038518No Hit
GCTGCGTTCTTCATCGATGCTCCCAGCCCCTGCCCCTACATCCTTCATCC1110.12835486071763086No Hit
GCTGCGTTCTTCATCGATGCCGCCAGGCAGACCTACGAATACGCCGAAGT1110.12835486071763086No Hit
GCTGCGTTCTTCATCGATGCCTCCGCGGGAGTGTGAGGCGGCACGTCGTG1100.12719851062107562No Hit
GCTGCGTTCTTCATCGATGCACACATGGGGGCCACGTCAGGTGACCACTC1090.1260421605245204No Hit
GCTGCGTTCTTCATCGATTCAAATAAAGTGAAGTGTCTAGACTTTAGATG1090.1260421605245204No Hit
GCTGCGTTCTTCATCGATGCCGGTGAATTATGCGATGGTGGTGGCGGCGG1080.12488581042796518No Hit
GCTGCGTTCTTCATCGATGCGGATTATCGGGGAGAAATCGGCGTAATCTT1040.12026041004174423No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC1030.119104059945189No Hit
GCTGCGTTCTTCATCGATGCACACAGGAGCTCAGAATATACCTAGTGAAG1020.11794770984863377No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAAGGGTTCGATGCGCGT1000.1156350096555233No Hit
GCTGCGTTCTTCATCGATGGCCGCGCCGCGGTCAAGGACGAACAACCAAT980.11332230946241284No Hit
GCTGCGTTCTTCATCGATGCTGGTCTTCGTCACTGTCTTCCTGGTCCTGC940.10869690907619192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)