FastQCFastQC Report
Sun 21 May 2023
EGAF00007833087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142649
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC8916362.5051700327377No Hit
GGAAGTAAAAGTCGTAACAAGAGATGGAGATTAGTCAATATTATCGAAAG74095.193867464896354No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC50263.523333496904991No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC49983.503704898036439No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG26851.8822424272164544No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC24991.7518524490182195No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT23651.6579155830044374No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT22781.5969267222342955No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAATCAAGT21981.5408450111812912No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC14771.0354085903160906No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC13130.9204410826574319No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC11670.8180919599856993No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA11440.8019684680579604No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC9370.656857040708312No Hit
GGAAGTAAAAGTCGTAACAAGGCCTATGAAGAACGCAGCGTCCTTGATAG8110.5685283457998304No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC7680.5383844261088405No Hit
GGAAGTAAAAGTCGTAACAAGGATAAAACCCAAAATAAGCCGGCAGTTGT7590.5320752336153777No Hit
GGAAGTAAAAGTCGTAACAAGGGCATAAATGTGAAGCGACGGGATTCGCT6910.484405779220324No Hit
GGAAGTAAAAGTCGTAACAAGGATCTTAGCGACTTGCTGCGTGAGATAGA6220.4360353034371079No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC5900.4136026190159061No Hit
GGAAGTAAAAGTCGTAACAAGACGAAGAAGTACCATGCTCACGACGAGAA5510.3862627848775666No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGGGGAAGGATC5320.3729433785024781No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA5130.3596239721273896No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC5040.3533147796339266No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG4950.34700558714046364No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC4470.3133565605086611No Hit
GGAAGTAAAAGTCGTAACAAGGGATGGAGATTAGTCAATATTATCGAAAG4180.2930269402519471No Hit
GGAAGTAAAAGTCGTAACAAGCGGTAAGAAGAATACAATCAACACTTGAC3600.252367699738519No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGGAGAGAAACGATGAGCCTGGAT3360.23554318642261773No Hit
GGAAGTAAAAGTCGTAACAAGGCCGATGAAGAACGCAGCGTCCTTGATAG3100.2173166303303914No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCGATGGTAGTTG3000.21030641644876588No Hit
GGAAGTAAAAGTCGTAACAACTGGAGGGGCGCGGCGCCGGTGGTGTGGAG2820.19768803146183989No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCTGTTGCGGGCAGGTGTCGTTTTGT2690.18857475341572671No Hit
GGAAGTAAAAGTCGTAACAAGGTTTACGTAGGTGAACCTGCGGAAGGATC2600.18226556092226376No Hit
GGAAGTAAAAGTCGTAACAAGTCCACAGGATTTAGTATCTATGGTAGTTG2420.1696471759353378No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCGTCCTTGAT2200.1542247053957616No Hit
GGAAGTAAAAGTCGTAACAAGGAACGCAGATGTATTATGCCAAGAAGGGC2020.14160632040883567No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1730.12127670015212164No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGCAGTTGTTTGGATACGCCGCGCGT1700.11917363598763397No Hit
GGAAGTAAAAGTCGTAACAAGAAAAGGCAGATGCTAGAGAATCATCTCGT1620.11356546488233356No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA1490.10445218683622039No Hit
GGAAGTAAAAGTCGTAACAAGGGTAGCAGTTGTTTGGATACGCCGCGCGT1490.10445218683622039No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)