FastQCFastQC Report
Sun 21 May 2023
EGAF00007833107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140942
Sequences flagged as poor quality0
Sequence length247-250
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC6437545.674816591221926No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4634632.88302989882363No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC63634.514623036426332No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC46303.2850392359977865No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA45273.2119595294518315No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG24991.7730697733819585No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC11990.8507045451320401No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC5890.41790239956861686No Hit
GGAAGTAAAAGTCGTAACAAGGATAAAACCCAAAATAAGCCGGCAGTTGT5620.3987455832895801No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAAGACAATAAAAAGACCTTCCGACG4860.3448226930226618No Hit
GGAAGTAAAAGTCGTAACAAGGAACTGTTGAAAAAGCTGGTAATTGTCAT3540.2511671467695932No Hit
GGAAGTAAAAGTCGTAACAAGGCGACCGTATGCTCGGAAAGCGGCGGAAT2790.19795376821671326No Hit
GGAAGTAAAAGTCGTAACAAGGCGAAGTCCGTGGTCAAGTACGCCTCCAG2730.19369669793248287No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2630.18660158079209888No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGAAGAAGGATC2460.17453988165344608No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG2230.15822111223056293No Hit
GGAAGTAAAAGTCGTAACAAGGGTTAATCAATGAAAACTATATGGGCATC2010.14261185452171815No Hit
GGAAGTAAAAGTCGTAACAAAGTTTTGGTAGGTGAACCTGTGGAGGGATC1750.1241645499567198No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC1750.1241645499567198No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTATGTGAACCTGCGGAAGGATC1700.12061699138652779No Hit
GGAAGTAAAAGTCGTAACAAGGTACCCGTACCGGAAGGTGCGGGTGGATC1610.1142313859601822No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1570.1113933391040286No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA1500.10642675710575981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)