FastQCFastQC Report
Sun 21 May 2023
EGAF00007833109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104403
Sequences flagged as poor quality0
Sequence length247-250
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA3971138.0362633257665No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1480214.17775351283009No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGATCCAGTCGAGCATCTCTCGACG93408.946103081329081No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC55315.297740486384491No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA43924.20677566736588No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA36973.541085984119231No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT12401.187705334137908No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC12191.167590969608153No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC12081.157054873902091No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC11661.1168261448425811No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC11131.0660613200770093No Hit
GGAAGTAAAAGTCGTAACAAGGCAGCCCATCTCAGCCACAAGTTGTCGGT9150.8764115973678916No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC9100.8716224629560454No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC7570.7250749499535454No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC6360.6091778971868624No Hit
GGAAGTAAAAGTCGTAACAAGGTTAGTCGCACCGTAACTTCCGACAATCA5370.5143530358323036No Hit
GGAAGTAAAAGTCGTAACAAGGATAATGCATTCAAGACATCCAGCCGATA4930.47220865300805537No Hit
GGAAGTAAAAGTCGTAACAAGGGAGAAAACGGTGAATGTGAGCGAAGAAG4720.45209428847830047No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTACGGCA3820.3658898690650652No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC3780.3620585615355881No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA3440.32949244753503254No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG3360.32182983247607827No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTTAC3300.31608287118186257No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC3230.3093780830052776No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGAAACCTCTCCGGCA2890.2768119690047221No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA2680.2566976044749672No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAACACA2680.2566976044749672No Hit
GGAAGTAAAAGTCGTAACAAGGGGGCGTAGCTGGCGATAGAGGTAGTCCT2600.24903498941601293No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA2580.2471193356512744No Hit
GGAAGTAAAAGTCGTAACAAGGATACCGCCACGGATATCCCTATCATCAC2350.22508931735678092No Hit
GGAAGTAAAAGTCGTAACAAGGCTGCCATTATCGGCCACTTCGGACTCGG2340.22413149047441167No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA2090.20018581841517963No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA2060.1973123377680718No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGAATGAGGCTTTTGCCGAGTTTAA1910.1829449345325326No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCGGCGGAGGGATC1910.1829449345325326No Hit
GGAAGTAAAAGTCGTAACAAGGGCCGAGAAGGAGAACTTGTTGCGGTCGT1890.18102928076779404No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA1850.1771979732383169No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC1740.16666187753225484No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAGATGAAGGACACCTCTCCGGCA1680.16091491623803914No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA1570.15037882053197704No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACTGAATCCGAAAAGAGCACA1500.14367403235539208No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGTAAACTGAATCCGAAAAGAGCACA1490.1427162054730228No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGATCCAGTCGAGCATCTCTCGAAG1480.14175837859065352No Hit
GGAAGTAAAAGTCGTAACAAGGTAGTGATGGACAAACCGGCGAAAAAGAC1420.13601141729643784No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGCAGCCACAGCAGTTGTTCGCGACG1410.13505359041406856No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1390.13313793664933002No Hit
GGAAGTAAAAGTCGTAACAAGCGGTAAGAAGAATACGATTAGCACTTGAC1330.12739097535511432No Hit
GGAAGTAAAAGTCGTAACAAGGGCGATGTGAAGACCGGATGCAATGCCAG1310.12547532159037575No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAGTGGAACTTTGCAGGGGATGAAAA1230.11781270653142151No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCAGGCA1200.11493922588431367No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGAACAGAGCTGAATATGGTCTTTCA1170.11206574523720583No Hit
GGAAGTAAAAGTCGTAACAAGGAGTACGTGAACGAGAAAGCACTGCTTTC1140.10919226459009798No Hit
GGAAGTAAAAGTCGTAACAAGGGAGAAAACTGTGAATGTGAGCGAAGAAG1100.10536095706062086No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC1090.10440313017825158No Hit
GGAAGTAAAAGTCGTAACAAGGGGCGTAAGTTCAAAGTCAATATCGTCTA1080.10344530329588231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)