FastQCFastQC Report
Sun 21 May 2023
EGAF00007833110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104403
Sequences flagged as poor quality0
Sequence length235-238
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT3785636.25949445897149No Hit
GCTGCGTTCTTCATCGAGGTAGTGTCGTGCTTCGGGAGGTTTTCCCAAAC86498.284244705611908No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA54315.201957798147562No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG44814.292022259896746No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT40753.9031445456548184No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT40083.8389701445360767No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT35083.3600567033514364No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG29402.8160110341656845No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT12341.1819583728436922No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT10731.0277482447822381No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT9920.9501642673103263No Hit
GCTGCGTTCTTCATCGAAGACGAAACGTTCGATGCGCTTACACGTAATGC8310.795954139248872No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG8000.7662615058954244No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT7810.7480627951304082No Hit
GCTGCGTTCTTCATCGATGTAGTGTCGTGCTTCGGGAGGTTTTCCCAAAC7730.7404001800714539No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTTGTCATTTACTACAAACAAATTTC7450.713581027365114No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT7180.6877197015411434No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT6880.6589848950700651No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT6450.617798339128186No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT5970.5718226487744605No Hit
GCTGCGTTCTTCATCGATGCCGATATGTTTCTCAAAATCACCCTGACCAT5500.5268047853031043No Hit
GCTGCGTTCTTCATCGATGCCTGCGGATGCAATTGCCTCGACACTCGGGT4800.4597569035372547No Hit
GCTGCGTTCTTCATCGATGCCATGAATCCCTGCAGATATCCAACCAGCTC4630.4434738465369769No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT4500.4310220970661763No Hit
GCTGCGTTCTTCATCGATGCCCGGCTTCCTTTTTCTCTTTATCGAAAAAA4160.39845598306562074No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG3940.37738379165349656No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGTCCGGACGATCGGGCAAACT3740.35822725400611094No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3730.35726942712374166No Hit
GCTGCGTTCTTCATCGATGCTTGGGAATAGGTTGCTGAACGAACGACTTT3730.35726942712374166No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG3350.320872005593709No Hit
GCTGCGTTCTTCATCGATGCCTTTGCCGAAGACGAACCGTGGCGGGCCGT3300.31608287118186257No Hit
GCTGCGTTCTTCATCGATGCGACTGAGCAGTAGAATGAGTGGAGAATATC3200.3065046023581698No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTGAAAGTTT3140.3007576410639541No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA3070.29405285288736915No Hit
GCTGCGTTCTTCATCGATGCTTGGGAATAGGTTGCTGAACGAACGACTCT3050.2921371991226306No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCTGACGATCGGGCAAACT3010.28830589159315345No Hit
GCTGCGTTCTTCATCGATGCGAAGTACAAGAGGTAATGCTCCAAAGCAAA2780.26627587329866004No Hit
GCTGCGTTCTTCATCGATGCTGGAACCAAGAGATCCGTTGTTGAAAGTTT2650.25382412382785935No Hit
GCTGCGTTCTTCATCGATGCTCATGGGGGACGATGTAGCTCCTCGCCGTG2470.2365832399452123No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCATTGTTAAAAGTTG2320.22221583670967307No Hit
GCTGCGTTCTTCATCGATGGTGGGATCTTCGGGGAGAGGCTCGTCGCTGA2300.2203001829449345No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA2290.21934235606256525No Hit
GCTGCGTTCTTCATCGATGTGCATCACGATGTCGGTACCACGGTCAGCCT2270.2174267022978267No Hit
GCTGCGTTCTTCATCGATGCGTTCAATATTGCTATTTACTTTGCAAATTA2200.21072191412124172No Hit
GCTGCGTTCTTCATCGATGCCTTTTCCGAGGCCGGACGATCGGGCAAACT2190.20976408723887247No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT2150.2059327797093953No Hit
GCTGCGTTCTTCATCGAGGTAGTGTCGTTCTTCGGGAGGTTTTCCCAAAC2150.2059327797093953No Hit
GCTGCGTTCTTCATCGATGCCAAATATACCGCCGTATCTATACTCCCCCC2080.19922799153281037No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGTACGATCGGGCAAACT2080.19922799153281037No Hit
GCTGCGTTCTTCATCGATGCGTGAGCCGAGATATCCGTTGCCGAGAGTCG2020.19348103023859467No Hit
GCTGCGTTCTTCATCGATGCACGCATCGCTGTGGGCAGCTGGAACACGTA1940.18581841517964043No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGTCAAACT1930.18486058829727114No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC1850.1771979732383169No Hit
GCTGCGTTCTTCATCGATGCCAGTTGGCGGGTGAATACTGTAATGTCTTC1810.1733666657088398No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGCT1770.16953535817936266No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA1740.16666187753225484No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCATTGTTGAAAGTTG1720.16474622376751627No Hit
GCTGCGTTCTTCATCGCTGTCAGATGCCTTGGCATCCTTCGTATGCCCTT1700.16283057000277767No Hit
CCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT1640.157083608708562No Hit
GCTGCGTTCTTCATCGAGGTAGTGTCGTGCTTCGGGATGTTTTCCCAAAC1580.15133664741434633No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTTCCGAGAGTCG1560.1494209936496078No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG1470.14080055170828423No Hit
GCTGCGTTCTTCATCGATGCCTTTCGCCGACAAACAGCTAACGTAGATAT1420.13601141729643784No Hit
GCTGCGTTCTTCATCGCTTCGAGAACGAGCAATTTCTGACCTTTCTTAAC1410.13505359041406856No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGTGCAAACT1390.13313793664933002No Hit
GCTGCGTTCTTCATCGATGTCGTTTCCGAGGCCGGACGATCGGGCAAACT1280.12260184094326791No Hit
GCTGCGTTCTTCATCGATGACGAAACGTTCGATGCGCTTACACGTAATGC1230.11781270653142151No Hit
GCTGCGTTCTTCATCGATGCCTTCCCGATTCGCCTGACAGGAGGACGAAG1220.11685487964905224No Hit
GCTGCGTTCTTCATCGATGCCTTTTCCTAGGCCGGACGATCGGGCAAACT1210.11589705276668295No Hit
GCTGCGTTCTTCATCGATGCAGGACGCTCGCCAGCATCTTGGCAGAAAGC1160.11110791835483654No Hit
GCTGCGTTCTTCATCGATGCTTCCTGCGTTAGCTTCGTACTGCTCCACTT1160.11110791835483654No Hit
GCTGCGTTCTTCATCGATGCCTCATCATAGTCGCCATAACCATGAAGGCT1130.1082344377077287No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCTAGGCCGGACGATCGGGCAAACT1090.10440313017825158No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGCTGAAAGTTT1080.10344530329588231No Hit
GCTGCGTTCTTCATCGATGCACTCAACAGTTGGCAACTTGTCAAGGAACT1070.10248747641351302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)