FastQCFastQC Report
Sun 21 May 2023
EGAF00007833111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89599
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA4473149.923548253886764No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1642618.33279389278898No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC76768.5670599002221No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC19242.147345394479849No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG17361.9375216241252693No Hit
GGAAGTAAAAGTCGTAACAAGCCAAAACCTGTTACATCAGTCATCGCTTT7900.8817062690431813No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC7490.835946829763725No Hit
GGAAGTAAAAGTCGTAACAAGGCTGAGGTCAAAGTTGCCGATAAGACTAT6280.7009006796950858No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCCAGTGCTTG6270.6997845958102211No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC6220.6942041763858972No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC6020.6718824986886015No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT5610.6261230594091451No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTACGAGTGCTGGAAGG5410.6038013817118495No Hit
GGAAGTAAAAGTCGTAACAAGGACAAGGCACGTCAGTATATGTGCGAGCA4710.5256755097713144No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA4460.49777341264969477No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT4040.45089788948537374No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG3770.42076362459402444No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC3720.41518320516970053No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC2850.3180839071864641No Hit
GGAAGTAAAAGTCGTAACAAGGATAATGCATTCAAGACATCCAGCCGATA2670.297994397258898No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG2480.27678880344646706No Hit
GGAAGTAAAAGTCGTAACAAGGTTTTCGTAGGTGAACCTGCGGAAGGATC2410.2689762162524135No Hit
GGAAGTAAAAGTCGTAACAAGCGGTAAGAAGAACACAATCAACACTTGAC2050.22879719639728124No Hit
GGAAGTAAAAGTCGTAACAAGGCTATGTAAAAAATGACGGTAATAATGGT1870.20870768646971505No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCAGGCA1740.19419859596647285No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA1740.19419859596647285No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1670.18638600877241934No Hit
GGAAGTAAAAGTCGTAACAAGGTGCAGCAATGTTATAACCGCGATTGGAT1630.18192167323296018No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTACGGCA1440.16071607942052926No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1410.1573678277659349No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA1350.1506713244567462No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC1340.14955524057188138No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1230.13727831783836875No Hit
GGAAGTAAAAGTCGTAACAAGGGTGGGGTGGATGGCCACAGGAGGAATGG1160.1294657306443152No Hit
GGAAGTAAAAGTCGTAACAAGGTAGGAGAAGAAGAGCCCAGGCGAGGCAA1100.1227692273351265No Hit
GGAAGTAAAAGTCGTAACAGTGCATATCCTTGTCTTCGCCTGCCATACGT1080.12053705956539694No Hit
GGAAGTAAAAGTCGTAACAAGGGAATAAGTATCGGCTACCTGCCGCAGGT1040.1160727240259378No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGGCATGAAACTCACTGAAAGCTAC990.11049230460161387No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC970.10826013683188428No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)