FastQCFastQC Report
Sun 21 May 2023
EGAF00007833122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73991
Sequences flagged as poor quality0
Sequence length235-238
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3364245.46769201659661No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG2174429.387357921909423No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT26293.553134840723872No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT18552.5070616696625265No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG15682.1191766566204No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA7881.0649943912097417No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG5680.7676609317349408No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT5320.7190063656390643No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT5150.6960305983160114No Hit
GCTGCGTTCTTCATCGATGCACGATCCTTCCTCGCTGCAGGATCCTTCCT4730.6392669378708221No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT4560.6162911705477693No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG3820.5162790069062453No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG3810.5149274911813599No Hit
GCTGCGTTCTTCATCGATTTCACCCAACAATGCTTCAGGAGAAACGACAA3780.5108729440067035No Hit
GCTGCGTTCTTCATCGATGCCGATATGTTTCTCAAAATCACCCTGACCAT3510.47438201943479613No Hit
GCTGCGTTCTTCATCGATGAGTGACTGTTGGGCCCCACTGCTGCGACTCT2150.2905758808503737No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC2040.27570920787663367No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT1920.2594910191780081No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCATTGTTGAAAGTTG1870.2527334405535808No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA1810.2446243462042681No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG1640.2216485788812153No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1630.2202970631563298No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAAC1610.2175940317065589No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT1460.197321295833277No Hit
GCTGCGTTCTTCATCGATGCCCGCATGCCTGTTCGTCGCTACGAGGTGCT1410.19056371720884974No Hit
GCTGCGTTCTTCATCGATGCGCACACTACTCCCACCGCCCCCGAGTGCCT1100.1486667297374005No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT1090.14731521401251504No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCTGGGAAAGTCA910.12298793096457677No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAAGGGTTCGATGCGCGT890.12028489951480587No Hit
TCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG860.11623035234014949No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT830.11217580516549311No Hit
GCTGCGTTCTTCATCGATGCCAGAGCCAAGAGATCCGTTGTTAAAAGTTT820.11082428944060765No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC780.10541822654106582No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT750.10136367936640943No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)