FastQCFastQC Report
Tue 7 May 2024
EGAF00007833131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65757
Total Bases16.3 Mbp
Sequences flagged as poor quality0
Sequence length247-250
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA2062631.367002752558665No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTCAAGGTTTCGGATAAAGCA896313.630488008881184No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC806912.270936934470855No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAAC37125.645026385023648No Hit
GGAAGTAAAAGTCGTAACAAGGGGAGGTGTGGTGTTCCAAGCAAGGCAGG33975.165989932630747No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC27984.2550602977629755No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC22433.4110436911659594No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT20953.185972596073422No Hit
GGAAGTAAAAGTCGTAACAAGGATGTTGCGTTAAGGTTTCGGATAAAGCA12851.9541645756345334No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA10161.5450826527974209No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC9411.4310263546086348No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT7791.1846647505208572No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG6520.9915294189211795No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC5890.8957221284425992No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA5050.767979074471159No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA2880.43797618504493824No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2760.4197271773347324No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2590.39387441641194093No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC2580.39235366576942377No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA2420.36802165548914945No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGTGTGGTGTTCCAAGCAAGGCAGG2110.3208783855711179No Hit
GGAAGTAAAAGTCGTAACAAGGCTGTAGGTCCCGACCTCGCCGTTGATGA1850.2813388688656721No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG1850.2813388688656721No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGCGTGGTGTTCCAAGCAAGGCAGG1750.2661313624405006No Hit
GGAAGTAAAAGTCGTAACAAGGACAATGTGTCGTTATCGATTACGGTATC1710.260048359870432No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACAACTCTCCGGCA1660.25244460665784635No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC1260.19161458095716047No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGAA1040.15815806682178324No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA1040.15815806682178324No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG1010.1535958148942318No Hit
GGAAGTAAAAGTCGTAACAAGGAACTTAGCGACTTGCTGCGTGAGATAGA970.1475128123241632No Hit
GGAAGTAAAAGTCGTAACAAGGCTAAGAAGGACTACAGAAAGAAACAGGG820.12470155268640602No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACATCTCCGGCA780.11861855011633743No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC690.1049317943336831No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGAAACCTCTCCGGCA680.10341104369116597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)