FastQCFastQC Report
Sun 21 May 2023
EGAF00007833144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85657
Sequences flagged as poor quality0
Sequence length235-238
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2272826.533733378474615No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG1395916.296391421600102No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA56366.579730786742473No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGGT50705.9189558354833816No Hit
GCTGCGTTCTTCATCGATGCTAGGCTTTTTACTTCTTCTTTTGCTTCAGC41094.797039354635348No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCCGGGAAGGTCA31393.664615851594149No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT17262.0150133672671235No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG16731.9531386810184805No Hit
GCTGCGTTCTTCATCGATGCGCCCTCAAAACTAGACAATGCAAAACTTTG16401.9146129329768728No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT13431.5678812006024025No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC12041.405606080063509No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCC8961.0460324316751697No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT8811.0285207280198934No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG8220.9596413603091399No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTTGTCATTTACTACAAACAAATTTC6810.7950313459495431No Hit
GCTGCGTTCTTCATCGATGCCATGAACCGTTTGCGCTTCGATAGCAGGCT6810.7950313459495431No Hit
GCTGCGTTCTTCATCGATGCTGGCTCCAGGGAGCTTCTTGATGAGGTCAG6480.7565055979079351No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT6010.7016355931214028No Hit
GCTGCGTTCTTCATCGATGCCGAAACCGAGCCCTTCGTGTATCAGCAGGT5430.6339236723210012No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGCT4630.540527919492861No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT4080.4763183394235147No Hit
GCTGCGTTCTTCATCGCAGCGACAGCCGTGAGCGCGGCGCCGTAGACATT3660.42728556918874117No Hit
GCTGCGTTCTTCATCGATGCTGTTTCAAACAGCAAAGTGGAGAATAAGGA3440.4016017371610026No Hit
GCTGCGTTCTTCATCGATGCCATTGAAAGTATACCAATAGCTTTCTCTAT2990.34906662619517376No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT2670.3117083250639177No Hit
GCTGCGTTCTTCATCGATGCCCTCCACTCCTTGTTCGCCGCGCAGGAGTT2570.3000338559604002No Hit
GCTGCGTTCTTCATCGATGCAGGTGAAGAGGTCTATGGCGGACAGGTCGT2510.2930291744982897No Hit
GCTGCGTTCTTCATCGATGCCGAGCATGCTTTTGACCCGATTTACGCCCG2430.28368959921547565No Hit
GCTGCGTTCTTCATCGATGCGACAGCCGTGAGCGCGGCGCCGTAGACATT2380.27785236466371693No Hit
GCTGCGTTCTTCATCGATGCTGTCCGCGTAATCGTAGATTGTCTTATGAC2360.2755174708430134No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTTCCGTCTTTTCTTCTTGTTT2260.2638430017394959No Hit
GCTGCGTTCTTCATCGATGTGTTTTGGTAATGGTAACTGAACCAAAATCC2210.2580057671877371No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT2140.2498336388152749No Hit
GCTGCGTTCTTCATCGTTTCGAAACGCCTAGGCATCCGCCGTATGCCCTT1890.22064746605648106No Hit
GCTGCGTTCTTCATCGATGCTACGAAGCGCCAGCGTTTTCCTTCGTTCTC1780.2078055500426118No Hit
GCTGCGTTCTTCATCGACCCCTCCAAGCTCAAGATCGCGGTTCTGTGTTC1740.2031357624012048No Hit
GCTGCGTTCTTCATCGATGCCAGTACCAAGTGCCTTACGGACACCACGAG1600.18679150565628028No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1540.17978682419416978No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT1530.17861937728381802No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG1530.17861937728381802No Hit
GCTGCGTTCTTCATCGATGCGCTCCCGTAGTAAGTCCTCGCTACCCAGTT1500.17511703655276278No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCTAGGCCGGACGATCGGGCAAACT1440.16811235509065225No Hit
GCTGCGTTCTTCATCGATGCGCCCTCAAAACTAGACAATGCAAAACTTGG1300.15176809834572771No Hit
GCTGCGTTCTTCATCGATGCCTTCCTTAACGTGGACAAAGGATTGGTCAA1280.14943320452502423No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC1230.14359596997326549No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACTAATGTGT1190.13892618233185847No Hit
GCTGCGTTCTTCATCGATGCGACCGAGGGTGAAATAGTTACTTGCAGGGT1160.1354238416008032No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT1160.1354238416008032No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGATGCCGGACGATCGGGCAAACT1150.13425639469045147No Hit
GCTGCGTTCTTCATCGATGCCATGAATCGCCTGCGCACGTCGGCCGACTT1150.13425639469045147No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCTGACGATCGGGCAAACT1040.12141447867658219No Hit
GCTGCGTTCTTCATCGATGCTCTGGCACTCCTTTCTCCATACGCTTTTGC1020.11907958485587868No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCCGGGAAAGTCA940.10974000957306466No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGTCCGGACGATCGGGCAAACT940.10974000957306466No Hit
GCTGCGTTCTTCATCGATGCCAAATATACCGCTGTATCTATACTCCCCCC920.10740511575236117No Hit
GCTGCGTTCTTCATCGATGCGCACGGCGACCTTGACGCTGACCTTTTCGA920.10740511575236117No Hit
GCTGCGTTCTTCATCGATGGTGAAGGCGGTGCGATCCCAATCGCACGAGC910.1062376688420094No Hit
GCTGCGTTCTTCATCGATGCGCCTGACAAGGATGCCATTGAGAAGAATAT910.1062376688420094No Hit
GCTGCGTTCTTCATCGATGCGCGAGTTACCTTGTTACGACGCTGAGACGA860.10040043429025065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)