FastQCFastQC Report
Sun 21 May 2023
EGAF00007833146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84894
Sequences flagged as poor quality0
Sequence length235-238
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT3533741.62484981270761No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT2074024.430466228473154No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG42505.006243079605155No Hit
GCTGCGTTCTTCATCGATGCCGATTTGTTTCTCAAAATCACCCTGACCAT32653.8459726246849013No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT25362.987254694089099No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAAGCCGGACGATCGGGCAAACT16701.9671590453977903No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT11501.354630480363748No Hit
GCTGCGTTCTTCATCGATGCCATCTGGCCATGGTTGAGGAGGGTGGGAGC9851.1602704549202534No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT8501.001248615921031No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA8340.9824015831507527No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG7590.8940561170400735No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA7520.8858105402030768No Hit
GCTGCGTTCTTCATCGAAGAAAGGCACTGGTAGGCCTAAGTGGACTTGAA7240.8528282328550898No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT5730.6749593610855891No Hit
GCTGCGTTCTTCATCGATGCAAACCTCGGCCGCACCCCAAGGCCTTGCTG5250.6184182627747544No Hit
GCTGCGTTCTTCATCGATAAAGTCGAGAGAAATTTGCTCCTGTCCGAGGT5170.6089947463896153No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGATATCCGTTGTTAAGAGTCG4060.4782434565458101No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCTAGATATCCGTTGCCGAGAGTCA3860.4546846655829623No Hit
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTT3730.4393714514571112No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA3680.43348175371639924No Hit
GCTGCGTTCTTCATCGATGCCCACGCCGGTACGCTCGGCACGCTCGGCGC2570.30273046387259406No Hit
GCTGCGTTCTTCATCGATACGAGAACCAAGAGATCCATTGTTAAAAGTTT2240.26385845878389524No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAAGGGTTCGATGCGCGT1530.18022475086578557No Hit
GCTGCGTTCTTCATCGATGCTGTCCATGGTCACGCGATGCTCTCCTCAAT1480.1743350531250736No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG1390.16373359719179212No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT1380.16255565764364974No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTG1260.14842038306594105No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG1230.14488656442151387No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG1210.1425306853252291No Hit
GCTGCGTTCTTCATCGATGCCGATATGTTTCTCAAAATCACCCTGACCAT1070.12603953165123566No Hit
TCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1030.1213277734586661No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT1030.1213277734586661No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC870.10248074068838788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)