FastQCFastQC Report
Wed 8 May 2024
EGAF00007833151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92015
Total Bases22.9 Mbp
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG3014532.7609628864859No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2439726.51415530076618No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC1412415.34967124925284No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA40854.439493560832473No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG27462.9842960386893442No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC16301.7714503070151606No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG15501.6845079606585882No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTACGAGTGCTGGAAGG6860.7455306200076075No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC6700.728142150736293No Hit
GGAAGTAAAAGTCGTAACAAGGTATCTGTAGGTGAACCTGCAGATGGATC5980.6498940390153779No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT5710.6205509971200348No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG5340.5803401619301202No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC4630.5031788295386622No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC4580.49774493289137645No Hit
GGAAGTAAAAGTCGTAACAAGGAAATGCGGATCCTCATGTTGGGCCTGGA3710.40319513122860406No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA2970.3227734608487747No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2960.3216866815193175No Hit
GGAAGTAAAAGTCGTAACAAGGGCGTGGGGCAGAAGGTGGAAAATGCGCG2420.2630005977286312No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC2400.2608270390697169No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCTAGACGCAGCACGCGCAAGTCGT2130.23148399717437376No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAGGTGGCGTCTCTGAGGGCAAAGCT2100.2282236591860023No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA1970.21409552790305927No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG1940.2108351899146878No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAGGGATC1590.17279791338368744No Hit
GGAAGTAAAAGTCGTAACTAAGAATGGGTTGCCTGCCAGCCCTGGCAGGT1580.17171113405423027No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGGTGGCGTCTTCTGCGAGGTCAGT1580.17171113405423027No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC1570.17062435472477314No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGCAGTCGGGGTACCAGTGCCCAGA1530.16627723740694453No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGAAGGAATTCAGAAGAGCCAGGGTG1370.14888876813563004No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA1200.13041351953485844No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1140.12389284355811551No Hit
GGAAGTAAAAGTCGTAACAAGTCCTTAATCGGCGGCAGCGCAGCAATGGA950.1032440362984296No Hit
GGAAGTAAAAGTCGTAACAAGGGGTGTCGGTGTAGGTCATTGGTTTTCCT940.10215725696897246No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)