FastQCFastQC Report
Sun 21 May 2023
EGAF00007833163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61850
Sequences flagged as poor quality0
Sequence length247-250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC1822329.46321746160065No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC1372422.18916734033953No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1175619.007275666936135No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC798912.916734033953112No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG26894.347615198059822No Hit
GGAAGTAAAAGTCGTAACAAGGAATCATCATGCAGTGAGGAAGGATCATG6991.130153597413096No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG5070.8197251414713015No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG4970.803556992724333No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT3930.635408245755861No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAGGGATC3320.5367825383993533No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC2710.43815683104284564No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC2630.4252223120452708No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2570.41552142279708976No Hit
GGAAGTAAAAGTCGTAACAAGGCAATAGCCCATGCGGGTGCGTAAATCTT2200.35569927243330635No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG2150.34761519805982216No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC1780.2877930476960388No Hit
GGAAGTAAAAGTCGTAACAAGGCAAGTTAATAATGCCGTCATCGCCCATC1050.16976556184316896No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA1020.16491511721907842No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAGCTGGCATCTCAACAATGACGG780.12611156022635409No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA690.11156022635408246No Hit
GGAAGTAAAAGTCGTAACAAGGGCAGAAGGAATTCAGAAGAGCCAGGGTG660.1067097817299919No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC650.10509296685529508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)