FastQCFastQC Report
Sun 21 May 2023
EGAF00007833175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54171
Sequences flagged as poor quality0
Sequence length247-250
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC2267241.85265178785697No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC560710.350556570858947No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA36476.732384486164183No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC21273.926455114360082No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT21153.904303040372155No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG19183.540639825737018No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG15852.9259197725720405No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA14932.7560872053312657No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC7831.4454228277122445No Hit
GGAAGTAAAAGTCGTAACAAGGGAAGGCGTGGTGTTCCAAGCAAGGCAGG5961.1002196747337136No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC5330.9839212862970962No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC4670.8620848793634971No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACTGAATCCGAAAAGAGCACA3520.6497941703125288No Hit
GGAAGTAAAAGTCGTAACAAGGAAGGAGCATATATGATTCAGCAGGAAAC3270.6036440161710139No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC3260.6017980100053534No Hit
GGAAGTAAAAGTCGTAACAAGGCTATAGGTACCGACCTCACCGTTGATGA2820.5205737387162873No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGCGGGATTTACACAGTTCTTTCGAC2660.4910376400657179No Hit
GGAAGTAAAAGTCGTAACAAGCGGCAAGAAGAATACAATCAACACTTGAC2620.4836536154030754No Hit
GGAAGTAAAAGTCGTAACAAGGAGGAAGTAGGCCAAGTTGAGCAGGATAT2270.4190433996049547No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCGGAAGGATC2200.40612135644533054No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCTAGACGCAGCACGCGCAAGTCGT2090.385815288623064No Hit
GGAAGTAAAAGTCGTAACAAGGAGAGCAGCCATAGCAGTTGTTTGCGACG1980.36550922080079745No Hit
GGAAGTAAAAGTCGTAACAAGGTGCAGCAATGTTATAACCGCGATTGGAT1860.3433571468128704No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCTAGACGCAGCACGCGCAAGTCGT1680.31012903583097967No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGTTGAAAAAGCTGGTAATTGTCAT1590.2935149803400343No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGATGAAGAACGCAGCTTACGTGCA1520.2805929371804102No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGTGAACCTGCGGTTGGATC1510.27874693101474957No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC1500.276900924849089No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC1490.2750549186834284No Hit
GGAAGTAAAAGTCGTAACAAGGTCGACAAGTTTATGGAGACCAGCGTCAA1460.26951690018644664No Hit
GGAAGTAAAAGTCGTAACAAGGTAGGAGAAGAAGAGCCCAGGCGAGGCAA1360.2510568385298407No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT1350.24921083236418012No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT1300.23998080153587714No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC1170.21598272138228944No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTTCGGAAGGATC1150.21229070905096825No Hit
GGAAGTAAAAGTCGTAACAAGGTTATTAACGAAAGCTCTGACCACTGCTT1130.20859869671964704No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGGAAACAGAATCCGAAAAGAGCACA1120.20675269055398646No Hit
GGAAGTAAAAGTCGTAACAAGGTTGATGGTAGTAAATGAATTATTACCAC980.18090860423473815No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCTTAGACGCAGCACGCGCAGTCGTA980.18090860423473815No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA970.17906259806907754No Hit
GGAAGTAAAAGTCGTAACAAGGCATGAAGACTGCAACACTTTCCAAGCAT960.17721659190341696No Hit
GGAAGTAAAAGTCGTAACAAGGAGGATCTTAGCACATGCACATAGAAACA860.15875653024681102No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCTAGACGCAGCACGCGCAGTCGTA850.15691052408115044No Hit
GGAAGTAAAAGTCGTAACAAGGATTTTGCGGTGGTTGCGCAGGTCGGTGC830.15321851174982926No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG810.14952649941850807No Hit
GGAAGTAAAAGTCGTAACAAGGAGGCGAGACGCTTTATAGTTTGGCACGT790.14583448708718685No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCTTAGACGCAGCACGCGCAAGTCGT790.14583448708718685No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCTTACCGGAAGGTGCGGCTGGATC780.14398848092152627No Hit
GGAAGTAAAAGTCGTAACAAGGTTAAGAGAGGGCCGTTTTCCTTATTAGG770.1421424747558657No Hit
GGAAGTAAAAGTCGTAACAAGGACTACAGAAAGAAACAGGGTCACAGACA750.1384504624245445No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG730.13475845009322332No Hit
GGAAGTAAAAGTCGTAACAAGGGTGACATCGTCGAGGTTTCGGAAGGCTA730.13475845009322332No Hit
GGAAGTAAAAGTCGTAACAAGGAGCTGAATGAGGCTTTTGCCGAGTTTAA700.12922043159624153No Hit
GGAAGTAAAAGTCGTAACAAGGATAGAGACTTGGTACGAGTGCTGGAAGG690.12737442543058092No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA650.11999040076793857No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCG630.11629838843661738No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCTAGACGCAGCACGCGCAGTCGTA580.1070683576083144No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA560.10337634527699323No Hit
GGAAGTAAAAGTCGTAACAAGGCAGGGTGGGAGGAGGTGCCAGGAATTTG550.10153033911133263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)