FastQCFastQC Report
Sun 21 May 2023
EGAF00007833176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54171
Sequences flagged as poor quality0
Sequence length237-238
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTTGTTGAAAGTTT1212622.384670764800354No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCG783514.463458307950749No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACATGTGT35616.573627955917373No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGGCCGGACGATCGGGCAAACT31735.857377563641062No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACATGCGTGT21373.9449151760166874No Hit
GCTGCGTTCTTCATCGATGCTGTTTAATTTTTCAAGAACGAGTTCGTAAT21083.8913809972125306No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAAGCGTACTCCACAACAGG18253.368961252330583No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAAGTAGCAG14792.7302431190120173No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGTTTTTTTCTTTACGCT13982.5807166195935096No Hit
GCTGCGTTCTTCATCGATGCAAGTGCACGTAAGCTGCGTTCTTCATCGAT13562.5031843606357644No Hit
GCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTT7741.4288087722212992No Hit
GCTGCGTTCTTCATCGATGCAAGTGCACGTAAGCTGCGTTCTTCATCGGC6261.1555998597035315No Hit
GCTGCGTTCTTCATCGATGCGATAAAGGTTGGCATCGTGTTTAATGGGTG6021.1112957117276772No Hit
GCTGCGTTCTTCATCGACGACAGTCTCGACTATCTCGAGCGAATCGACGA5461.0079193664506838No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAAGGGTTCGATGCGCGT5400.9968433294567205No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT5060.9340791198242602No Hit
GCTGCGTTCTTCATCGACTCTTACTACCTAGGCATCCTTTGTGTGCCCTT3740.6904063059570619No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCGTTGCCGAGAGTCA3670.6774842627974377No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGCT3640.671946244300456No Hit
GCTGCGTTCTTCATCGATGAAGTGCACGTAAGCTGCGTTCTTCATCGATG3460.6387181333185653No Hit
GCTGCGTTCTTCATCGATGGGAGAACCAAGAGATCCGTTGTCGAAAGTTG3190.5888759668457293No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCA3170.5851839545144081No Hit
GCTGCGTTCTTCATCGGCGCAGTCCTTGTCATTTACTACAAACAAATTTC2900.5353417880415721No Hit
GCTGCGTTCTTCATCGATGCAGGTATCGAGCATGCCCTCAAGGCCTTCGA2870.5298037695445903No Hit
GCTGCGTTCTTCATCGATGCCTCATCATAGTCGCCATAACCATGAAGGCT2620.4836536154030754No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCTGAAAGTTG2590.4781155969060937No Hit
GCTGCGTTCTTCATCGATGCCAGGCAAACAAAGGAACAACACACACGTGT2520.46519355374646953No Hit
GCTGCGTTCTTCATCGATGCGCCCTCAAAACTAGACAATGCAAAACGTAG2420.44673349208986357No Hit
GCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTTGAAAGTTT2330.4301194365989182No Hit
GCTGCGTTCTTCATCGATGCTTTTCAGTGCGTGCCCTTCGCACATCTGCG2080.3839692824574034No Hit
GCTGCGTTCTTCATCGATGCAAGAGCCGAGATATCCGTTGCCGAGAGTCG2000.36920123313211867No Hit
GCTGCGTTCTTCATCGATGCCTTTCGCCGACAAACAACTAACGTAGATAT1980.36550922080079745No Hit
GCTGCGTTCTTCATCGATTCGAGAGCCGAGATATCCGTTGCCGAGAGTCG1950.3599712023038157No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCGAGATATCCTTTGCCGAGAGTCG1950.3599712023038157No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGAGTCCGGACGATCGGGCAAACT1770.326743091321925No Hit
GCTGCGTTCTTCATCGATGCCGTTTCCGAGGCCGGACGATCGGGCAAACT1760.3248970851562644No Hit
GCTGCGTTCTTCATCGATGTCTTCCTTTGTAGCATCAGCTGGGAAAGTCA1570.28982296800871316No Hit
GCTGCGTTCTTCATCGATGAAGTGCACGTAAGCTGCGTTCTTCATCGGCT1550.286130955677392No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGCCAAAAGTTG1540.2842849495117314No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTT1430.2639788816894648No Hit
GCTGCGTTCTTCATCGATGCTCATCAGGGATATTGGCCTAAAATTTTCTT1410.26028686935814366No Hit
GCTGCGTTCTTCATCGATGCGGACATCTCCGTACTTCTCACGTGCCTGGT1400.25844086319248305No Hit
GCTGCGTTCTTCATCGAAACGGATATAACTACCATCTTTTCTTCTGGTCT1340.2473648261985195No Hit
GCTGCGTTCTTCATCGAAACGGATATAGCTACCATCTTTTCTTCTGGTCT1330.24551882003285894No Hit
GCTGCGTTCTTCATCGATGCCGCTTCCGATGCCGGACGATCGGGCAAACT1250.23075077070757416No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAAGGGTTTGATCATGGC1190.21967473371361063No Hit
GCTGCGTTCTTCATCGATGCGCGTGCTGCGTCTAAGGGTTTGATTATGGC1180.21782872754795No Hit
GCTGCGTTCTTCATCGATGCGTCGATATTTCACTTCGCAAATCAAAATAA1170.21598272138228944No Hit
GCTGCGTTCTTCATCGATGCTGCTCAGCACAGGCCTGTTTCCGAGCCAGT1130.20859869671964704No Hit
GCTGCGTTCTTCATCGATGCTAGGTTTTTTACTTCTTCTTTTGCTTCAGC1100.20306067822266527No Hit
GCTGCGTTCTTCATCGATAAAGAGTACACCACCAATCGCAGACTTAATGA1020.18829262889738052No Hit
GCTGCGTTCTTCATCGATGCACCTTTCAATCTTAAATACTACAAAAAGGG1010.18644662273171994No Hit
GCTGCGTTCTTCATCGATGCCGTTCAGGTAGCAGGAAAGCTTCCTATTCA1000.18460061656605933No Hit
GCTGCGTTCTTCATCGAGGTCGATGAAGGAGTCATAGCGCTCGGAGCGCA1000.18460061656605933No Hit
GCTGCGTTCTTCATCGATGCTGGAACCAAGAGATCCGTTGTTGAAAGTTT990.18275461040039873No Hit
GCTGCGTTCTTCATCGATGCAGCCAGACAAATATGCTCTGTGGCAATGGC970.17906259806907754No Hit
GCTGCGTTCTTCATCGATGCAGACCTGTGTCTACCCTCGTGTGACACTGG940.17352457957209577No Hit
GCTGCGTTCTTCATCGATGCCGGTAGCTTTCATAGGCAGTAGAAAAGCTA890.1642945487437928No Hit
GCTGCGTTCTTCATCGATGCCAGTTGGCGGGTGAACACTGTAATGTCTTC870.1606025364124716No Hit
GCTGCGTTCTTCATCGATGCTGCCAGACAAATATGCTCAGTGGCAATGGC810.14952649941850807No Hit
GCTGCGTTCTTCATCGATGCCATCATCGACAGCATGCTTGCAGACATACG800.14768049325284746No Hit
GCTGCGTTCTTCATCGATGCCCAGCGTGAGCTGCATGATGATGAGCTTCT770.1421424747558657No Hit
GCTGCGTTCTTCATCGATGCGAGAGCCAAGAGATCCGTTGTTGAAAGTTG760.1402964685902051No Hit
GCTGCGTTCTTCATCGATGCAGTGCACGTAAGCTGCGTTCTTCATCGATG730.13475845009322332No Hit
GCTGCGTTCTTCATCGATGCCCCTTCCTTCCCATGCCTTAGCTTCCTCCT710.13106643776190213No Hit
GCTGCGTTCTTCATCGATGCGCACGGCGACCTTGACGCTGACCTTTTCGA700.12922043159624153No Hit
GCTGCGTTCTTCATCGATGCATATGTGGTGTGGCCCACATTTCTGTATGC670.12368241309925974No Hit
GCTGCGTTCTTCATCGATGCTTCTCAGTCCAAATCGTACTCCACAACAGG670.12368241309925974No Hit
GCTGCGTTCTTCATCGATGCGTTTTTGTTCTCTGCTTTTTTCTTTACGGT610.11260637610529618No Hit
GCTGCGTTCTTCATCGATGCAGAGCCTGACATCGCGCGGGCAGATCATAC570.10522235144265382No Hit
GCTGCGTTCTTCATCGATGCCCAGCGTGAGCTGCATGATGACGAGCTTCT560.10337634527699323No Hit
GCTGCGTTCTTCATCGATGCGTAGACAGACGAAGCACAGCCAATTAGCAG550.10153033911133263No Hit
GCTGCGTTCTTCATCGATGCCATATTCCAGTTTGACACATCAGGGTTTGC550.10153033911133263No Hit
GCTGCGTTCTTCATCGATGCAAGTGCACGTAAGCTGCGTTCTTCATCTGC550.10153033911133263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)