FastQCFastQC Report
Sun 21 May 2023
EGAF00007833183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007833183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89637
Sequences flagged as poor quality0
Sequence length247-250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC4323348.23119916998561No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATC62346.9547173600187415No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATC60386.7360576547631No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATC57716.438189586889342No Hit
GGAAGTAAAAGTCGTAACAAGGACATTCAAAAGGGGAGCGCGCGGAAGTG43294.829478898222832No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATC33673.7562613652844252No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACGGGAACGTGCGGATGGATC21472.3952162611421626No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCGAATCTAATAATGAAGAATCAG17791.984671508417283No Hit
GGAAGTAAAAGTCGTAACAAGGAACGAAGATGAAGGACACCTCTCCGGCA17731.9779778439706817No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATC8360.93265057955978No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGTTTGGTACACGTTTCCGAA8170.9114539754788759No Hit
GGAAGTAAAAGTCGTAACAAGGAAAACAGTGAACAGCCTCAGTTCCCTGG7990.891372982139072No Hit
GGAAGTAAAAGTCGTAACAAGGTATCAGTAGGTGAACCTGCTGATGGATC4850.5410712094336044No Hit
GGAAGTAAAAGTCGTAACAAGGCCCACCAAATCGGGATATCGGCACTCAC4800.5354931557281033No Hit
GGAAGTAAAAGTCGTAACAACACTAAATCTTATCTCCAGCCCTCAGAAGG4570.5098341086827984No Hit
GGAAGTAAAAGTCGTAACAAGGTCTCCTTTGGTGAACCAGCGGAGGGATC4100.45740040385108827No Hit
GGAAGTAAAAGTCGTAACAAGGTTGGGGGTAGTTTTGGGAAAGGCTATTG4000.44624429644008606No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAAGTGAAATTCCTGAATAGACCAAT3080.3436081082588663No Hit
GGAAGTAAAAGTCGTAACAAGGTATCTGTAGGTGAACCTGCAGATGGATC2970.33133639010676397No Hit
GGAAGTAAAAGTCGTAACAAGGAAAAGTGGGATAGTTTCTTCTTTGGAGA2720.30344612157925854No Hit
GGAAGTAAAAGTCGTAACAAGAGCAGAAGGAATTCAGAAGAGCCAGGGTG2660.2967524571326573No Hit
GGAAGTAAAAGTCGTAACAAGGATAACGTGGAATTTGGGACTCAGCCAAC2530.2822495174983545No Hit
GGAAGTAAAAGTCGTAACAAGGGATAACTCATGGCTCAAGCAGAGCAAAA2450.2733246315695527No Hit
GGAAGTAAAAGTCGTAACAAGGGCTGGCAGGGGCTGTGGGAGGAGTTTGT2430.2710934100873523No Hit
GGAAGTAAAAGTCGTAACAAGGAGGGGATCGTCGGCGGGTGCGGCCTTTC2070.23093142340774456No Hit
GGAAGTAAAAGTCGTAACTAAGAATGGGTTGCCTGCCAGCCCTGGCAGGT1840.2052723763624396No Hit
GGAAGTAAAAGTCGTAACAAGGCTCAAGTTGAAAAACGCTTGAACCAAGT1610.17961332931713467No Hit
GGAAGTAAAAGTCGTAACAAACAGGGAGTTGTGGTGGGAAGGAGCTTGCA1590.17738210783493422No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATC1350.15060745004852907No Hit
GGAAGTAAAAGTCGTAACAAGGTAGAGAAAATGAAGAAATAAAGGTTGGT1260.14056695337862712No Hit
GGAAGTAAAAGTCGTAACAAGGAAAGAAAACAGAATCCGAAAAGAGCACA1250.1394513426375269No Hit
GGAAGTAAAAGTCGTAACAAGGTAGCCGTACGGGAACGTGCGGCTGGATC1070.11937034929772304No Hit
GGAAGTAAAAGTCGTAACAAGGGCCAGCCAGGGAGAGGTCTCCCCAGGCC1050.1171391278155226No Hit
GGAAGTAAAAGTCGTAACAAGGGCGAGGAGTTTGCGGTTTTGTTCTTCTT1040.1160235170744224No Hit
GGAAGTAAAAGTCGTAACAAGCCACAGAAGAAATGAACGATCGTAGGGAT1040.1160235170744224No Hit
GGAAGTAAAAGTCGTAACAAGGTGTACATAAAAGGCGACGGCGGAAAATG1030.11490790633332218No Hit
GGAAGTAAAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGAAGGATC1020.11379229559222197No Hit
GGAAGTAAAAGTCGTAACAAGGGACAAGTGAAAGGAGGCTGGGGCAGCTG1000.11156107411002152No Hit
GGAAGTAAAAGTCGTAACAAGGTTTCTGTAGGTGAACCTGCAGAAGGATC990.11044546336892132No Hit
GGAAGTAAAAGTCGTAACAAGGAAGAGGGCGAGGAAAATGGTGAGGGAGA970.10821424188672088No Hit
GGAAGTAAAAGTCGTAACAAGGTATCCGTACCGGAAGGTGCGGATGGATC920.1026361881812198No Hit
GGAAGTAAAAGTCGTAACAAGGCATCGAAGGATTGGTACACGTTTCCGAA910.10152057744011958No Hit
GGAAGTAAAAGTCGTAACAAGGCAAAAATAGACAAGTGGGACTATATCAA910.10152057744011958No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)